Analysis of ATF3, a transcription factor induced by physiological stresses and modulated by gadd153/Chop10

Benjamin P C Chen, Curt D. Wolfgang, Tsonwin Hai

Research output: Contribution to journalArticle

236 Citations (Scopus)

Abstract

We demonstrate that ATF3, a member of the ATF/CREB family of transcription factors, is induced in a variety of stressed tissues: mechanically injured liver, toxin-injured liver, blood-deprived heart, and postseizure brain. We also demonstrate that an ATF3-interacting protein, gadd153/Chop10, forms a nonfunctional heterodimer with ATF3: the heterodimer, in contrast to the ATF3 homodimer, does not bind to the ATF/cyclic AMP response element consensus site and does not repress transcription. Interestingly, ATF3 and gadd153/Chop10 are expressed in inverse but overlapping manners during the liver's response to carbon tetrachloride (CCl4): the level of gadd153/Chop10 mRNA is high in the normal liver and greatly decreases upon CCl4 treatment; the level of ATF3 mRNA, on the other hand, is low in the normal liver and greatly increases upon CCl4 treatment. We hypothesize that in nonstressed liver, gadd153/Chop10 inhibits the limited amount of ATF3 by forming an inactive heterodimer with it, whereas in CCl4-injured liver, the synthesis of gadd153/Chop10 is repressed, allowing the induced ATF3 to function.

Original languageEnglish (US)
Pages (from-to)1157-1168
Number of pages12
JournalMolecular and Cellular Biology
Volume16
Issue number3
StatePublished - Mar 1996

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Activating Transcription Factor 3
Physiological Stress
Liver
Messenger RNA
Carbon Tetrachloride
Response Elements
Cyclic AMP
Transcription Factors

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Analysis of ATF3, a transcription factor induced by physiological stresses and modulated by gadd153/Chop10. / Chen, Benjamin P C; Wolfgang, Curt D.; Hai, Tsonwin.

In: Molecular and Cellular Biology, Vol. 16, No. 3, 03.1996, p. 1157-1168.

Research output: Contribution to journalArticle

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