Analysis of big endothelin-1 digestion by cathepsin D

Tatsuya Sawamura, Osamu Shinmi, Naoya Kishi, Yoshiki Sugita, Masashi Yanagisawa, Katsutoshi Goto, Tomoh Masaki, Sadao Kimura

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Digestion of big endothelin(ET)-1 by cathepsin D, which is the only substantially identified protease showing ET converting enzyme activity, was characterized. Increased doses of cathepsin D showed decrease of immunoreactive (ir-) ET produced from big ET-1. Time course of big ET-1 conversion showed marked increase of ir-ET in a relatively short period, but further incubation resulted in the decrease of ir-ET. Incubation at various pHs with different doses of cathepsin D revealed that low doses of cathepsin D yielded the maximum production of ir-ET at pH 3.5 - 4.0, but higher doses of cathepsin D showed a bimodal curve of ir-ET production, which may be due to degradation of ir-ET. HPLC analysis revealed that cathepsin D cleaves Asn18-Ile19 bond in addition to Trp21-Val22 bond of big ET-1. These data suggests cathepsin D is not a physiological endothelin converting enzyme.

Original languageEnglish (US)
Pages (from-to)883-889
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume172
Issue number2
DOIs
StatePublished - Oct 30 1990

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Cathepsin D
Endothelins
Endothelin-1
Digestion
Enzyme activity
Peptide Hydrolases
High Pressure Liquid Chromatography
Degradation
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Analysis of big endothelin-1 digestion by cathepsin D. / Sawamura, Tatsuya; Shinmi, Osamu; Kishi, Naoya; Sugita, Yoshiki; Yanagisawa, Masashi; Goto, Katsutoshi; Masaki, Tomoh; Kimura, Sadao.

In: Biochemical and Biophysical Research Communications, Vol. 172, No. 2, 30.10.1990, p. 883-889.

Research output: Contribution to journalArticle

Sawamura, T, Shinmi, O, Kishi, N, Sugita, Y, Yanagisawa, M, Goto, K, Masaki, T & Kimura, S 1990, 'Analysis of big endothelin-1 digestion by cathepsin D', Biochemical and Biophysical Research Communications, vol. 172, no. 2, pp. 883-889. https://doi.org/10.1016/0006-291X(90)90758-F
Sawamura, Tatsuya ; Shinmi, Osamu ; Kishi, Naoya ; Sugita, Yoshiki ; Yanagisawa, Masashi ; Goto, Katsutoshi ; Masaki, Tomoh ; Kimura, Sadao. / Analysis of big endothelin-1 digestion by cathepsin D. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 172, No. 2. pp. 883-889.
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