Analysis of constitutive EGFR signaling regulating IRF3 transcriptional activity in cancer cells

Gao Guo, Ke Gong, Amyn A. Habib

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Epidermal growth factor receptor (EGFR) plays an important role in various types of human cancers. Overexpression of EGFR leads to a constitutive tyrosine phosphorylation of multiple tyrosine residues in the EGFR. Recently, we have demonstrated that overexpressed EGFR oscillates between two distinct and mutually exclusive modes of signaling depending on the presence or absence of ligand. EGFR constitutively activates transcription factor IRF3, which results in transcription of its target genes. Addition of EGF causes a loss of IRF3 transcriptional activity and activation of canonical signaling pathways such as ERK. The mechanistic basis of this bimodal signaling appears to be the association of a distinct set of signaling proteins with EGFR in the absence or presence of ligand. In this chapter, we describe a detailed protocol for analyses of constitutive EGFR signaling with a focus on IRF3 target genes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages183-189
Number of pages7
Volume1652
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1652
ISSN (Print)1064-3745

Keywords

  • Bimodal signaling
  • Chromatin immunoprecipitation (ChIP)
  • Epidermal growth factor receptor (EGFR)
  • IRF3

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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