Analysis of dermal elastic fibers in the absence of fibulin-5 reveals potential roles for fibulin-5 in elastic fiber assembly

Jiwon Choi, Andreas Bergdahl, Qian Zheng, Barry Starcher, Hiromi Yanagisawa, Elaine C. Davis

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Fibulin-5 is a 66 kDa modular, extracellular matrix protein that localizes to elastic fibers. Although in vitro protein-protein binding studies have shown that fibulin-5 binds many proteins involved in elastic fiber formation, the specific role of fibulin-5 in elastogenesis remains unclear. To provide a more detailed analysis of elastic fiber assembly in the absence of fibulin-5, the dermis of wild-type and fibulin-5 gene knockout (Fbln5-/-) mice was examined with electron microscopy (EM). Although light microscopy showed apparently normal elastic fibers near the hair follicles and the absence of elastic fibers in the intervening dermis of the Fbln5-/- mouse, EM revealed the presence of aberrantly assembled elastic fibers in both locales. Instead of the elastin being incorporated into the microfibrillar scaffold, the elastin appeared as globules juxtaposed to the microfibrils. Desmosine analysis showed significantly lower levels of mature cross-linked elastin in the Fbln5-/- dermis, however, gene expression levels for tropoelastin and fibrillin-1, the major elastic fiber components, were unaffected. Based on these results, the nature of tropoelastin cross-linking was investigated using domain specific antibodies to lysyl oxidase like-1 (LOXL-1). Immunolocalization with an antibody to the N-terminal pro-peptide, which is cleaved to generate the active enzyme, revealed abundant staining in the Fbln5-/- dermis and no staining in the wild-type dermis. Overall, these results suggest two previously unrecognized functions for fibulin-5 in elastogenesis; first, to limit the extent of aggregation of tropoelastin monomers and/or coacervates and aid in the incorporation of elastin into the microfibril bundles, and second, to potentially assist in the activation of LOXL-1.

Original languageEnglish (US)
Pages (from-to)211-220
Number of pages10
JournalMatrix Biology
Volume28
Issue number4
DOIs
StatePublished - May 2009

Fingerprint

Elastic Tissue
Dermis
Elastin
Tropoelastin
Skin
Protein-Lysine 6-Oxidase
Microfibrils
Electron Microscopy
Desmosine
Staining and Labeling
Gene Knockout Techniques
Hair Follicle
Antibodies
Extracellular Matrix Proteins
fibulin
Protein Binding
Knockout Mice
Microscopy
Proteins
Gene Expression

Keywords

  • Elastic fibers
  • Elastin
  • Fibulin-5
  • Lysyl oxidase like-1
  • Skin

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Analysis of dermal elastic fibers in the absence of fibulin-5 reveals potential roles for fibulin-5 in elastic fiber assembly. / Choi, Jiwon; Bergdahl, Andreas; Zheng, Qian; Starcher, Barry; Yanagisawa, Hiromi; Davis, Elaine C.

In: Matrix Biology, Vol. 28, No. 4, 05.2009, p. 211-220.

Research output: Contribution to journalArticle

Choi, Jiwon ; Bergdahl, Andreas ; Zheng, Qian ; Starcher, Barry ; Yanagisawa, Hiromi ; Davis, Elaine C. / Analysis of dermal elastic fibers in the absence of fibulin-5 reveals potential roles for fibulin-5 in elastic fiber assembly. In: Matrix Biology. 2009 ; Vol. 28, No. 4. pp. 211-220.
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abstract = "Fibulin-5 is a 66 kDa modular, extracellular matrix protein that localizes to elastic fibers. Although in vitro protein-protein binding studies have shown that fibulin-5 binds many proteins involved in elastic fiber formation, the specific role of fibulin-5 in elastogenesis remains unclear. To provide a more detailed analysis of elastic fiber assembly in the absence of fibulin-5, the dermis of wild-type and fibulin-5 gene knockout (Fbln5-/-) mice was examined with electron microscopy (EM). Although light microscopy showed apparently normal elastic fibers near the hair follicles and the absence of elastic fibers in the intervening dermis of the Fbln5-/- mouse, EM revealed the presence of aberrantly assembled elastic fibers in both locales. Instead of the elastin being incorporated into the microfibrillar scaffold, the elastin appeared as globules juxtaposed to the microfibrils. Desmosine analysis showed significantly lower levels of mature cross-linked elastin in the Fbln5-/- dermis, however, gene expression levels for tropoelastin and fibrillin-1, the major elastic fiber components, were unaffected. Based on these results, the nature of tropoelastin cross-linking was investigated using domain specific antibodies to lysyl oxidase like-1 (LOXL-1). Immunolocalization with an antibody to the N-terminal pro-peptide, which is cleaved to generate the active enzyme, revealed abundant staining in the Fbln5-/- dermis and no staining in the wild-type dermis. Overall, these results suggest two previously unrecognized functions for fibulin-5 in elastogenesis; first, to limit the extent of aggregation of tropoelastin monomers and/or coacervates and aid in the incorporation of elastin into the microfibril bundles, and second, to potentially assist in the activation of LOXL-1.",
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