TY - JOUR
T1 - Analysis of expressed sequence tags from Trypanosoma cruzi amastigotes
AU - Cerqueira, Gustavo C.
AU - DaRocha, Wanderson D.
AU - Campos, Priscila C.
AU - Zouain, Cláudia S.
AU - Teixeira, Santuza M.R.
PY - 2005/7
Y1 - 2005/7
N2 - A total of 880 expressed sequence tags (EST) originated from clones randomly selected from a Trypanosoma cruzi amastigote cDNA library have been analyzed. Of these, 40% (355 ESTs) have been identified by similarity to sequences in public databases and classified according to functional categorization of their putative products. About 11% of the mRNAs expressed in amastigotes are related to the translational machinery, and a large number of them (9% of the total number of clones in the library) encode ribosomal proteins. A comparative analysis with a previous study, where clones from the same library were selected using sera from patients with Chagas disease, revealed that ribosomal proteins also represent the largest class of antigen coding genes expressed in amastigotes (54% of all immunoselected clones). However, although more than thirty classes of ribosomal proteins were identified by EST analysis, the results of the immunoscreening indicated that only a particular subset of them contains major antigenic determinants recognized by antibodies from Chagas disease patients.
AB - A total of 880 expressed sequence tags (EST) originated from clones randomly selected from a Trypanosoma cruzi amastigote cDNA library have been analyzed. Of these, 40% (355 ESTs) have been identified by similarity to sequences in public databases and classified according to functional categorization of their putative products. About 11% of the mRNAs expressed in amastigotes are related to the translational machinery, and a large number of them (9% of the total number of clones in the library) encode ribosomal proteins. A comparative analysis with a previous study, where clones from the same library were selected using sera from patients with Chagas disease, revealed that ribosomal proteins also represent the largest class of antigen coding genes expressed in amastigotes (54% of all immunoselected clones). However, although more than thirty classes of ribosomal proteins were identified by EST analysis, the results of the immunoscreening indicated that only a particular subset of them contains major antigenic determinants recognized by antibodies from Chagas disease patients.
KW - Amastigotes
KW - Antigens
KW - Gene expression
KW - Trypanosoma cruzi
UR - http://www.scopus.com/inward/record.url?scp=23944485908&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=23944485908&partnerID=8YFLogxK
U2 - 10.1590/S0074-02762005000400008
DO - 10.1590/S0074-02762005000400008
M3 - Article
C2 - 16113886
AN - SCOPUS:23944485908
SN - 0074-0276
VL - 100
SP - 385
EP - 389
JO - Memorias do Instituto Oswaldo Cruz
JF - Memorias do Instituto Oswaldo Cruz
IS - 4
ER -