TY - JOUR
T1 - Analysis of IFT kinesins in developing zebrafish cone photoreceptor sensory cilia.
AU - Insinna, Christine
AU - Luby-Phelps, Katherine
AU - Link, Brian A.
AU - Besharse, Joseph C.
N1 - Funding Information:
The authors wish to thank Dr Don Arnold for the DNKIF17 (USC, Los Angeles, CA), Dr Beth Burnside (UC Berkeley, CA) for the DNKIF3B constructs, and Dr Breandan Kennedy (UCD, Ireland) for the Zop and Ta-CP promoters. We also thank Clive Wells for assistance with EM imaging and Monica Humby for technical assistance. This work was supported by NIH grant EY03222 (JCB) and an NIH core grant for Vision Research. C. Insinna was supported by NIH NRSA Training grant T32-EY014537 .
PY - 2009
Y1 - 2009
N2 - The photoreceptor outer segment (OS), a well-defined sensory cilium, provides an important context for the study of intraflagellar transport (IFT). The early phases of OS development involve successive events that are common to virtually all cilia. Additionally, intense protein trafficking occurs through the cilium and relies on IFT to maintain proper cellular morphology and optimize the photosensitive function. In the past decade, progress has been made in the characterization of photoreceptor OS trafficking in murine and amphibian models. Recently, powerful and cost-effective molecular tools and techniques for zebrafish have opened new opportunities to study photoreceptor IFT. Studies using zebrafish take advantage of its rapid embryogenesis to characterize the early events involved in photoreceptor ciliogenesis and OS assembly. In this overview, we describe phenotypes associated with knockdown strategies or genetic mutations of IFT components in zebrafish and detail a general experimental approach that has enabled us to study the function of the two anterograde IFT motors, KIF17 and kinesin II, in zebrafish cone photoreceptors. 2009 Elsevier Inc. All rights reserved.
AB - The photoreceptor outer segment (OS), a well-defined sensory cilium, provides an important context for the study of intraflagellar transport (IFT). The early phases of OS development involve successive events that are common to virtually all cilia. Additionally, intense protein trafficking occurs through the cilium and relies on IFT to maintain proper cellular morphology and optimize the photosensitive function. In the past decade, progress has been made in the characterization of photoreceptor OS trafficking in murine and amphibian models. Recently, powerful and cost-effective molecular tools and techniques for zebrafish have opened new opportunities to study photoreceptor IFT. Studies using zebrafish take advantage of its rapid embryogenesis to characterize the early events involved in photoreceptor ciliogenesis and OS assembly. In this overview, we describe phenotypes associated with knockdown strategies or genetic mutations of IFT components in zebrafish and detail a general experimental approach that has enabled us to study the function of the two anterograde IFT motors, KIF17 and kinesin II, in zebrafish cone photoreceptors. 2009 Elsevier Inc. All rights reserved.
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U2 - 10.1016/S0091-679X(08)93012-0
DO - 10.1016/S0091-679X(08)93012-0
M3 - Article
C2 - 20409820
AN - SCOPUS:77954674137
VL - 93
SP - 219
EP - 234
JO - Methods in Cell Biology
JF - Methods in Cell Biology
SN - 0091-679X
ER -