Abstract
The photoreceptor outer segment (OS), a well-defined sensory cilium, provides an important context for the study of intraflagellar transport (IFT). The early phases of OS development involve successive events that are common to virtually all cilia. Additionally, intense protein trafficking occurs through the cilium and relies on IFT to maintain proper cellular morphology and optimize the photosensitive function. In the past decade, progress has been made in the characterization of photoreceptor OS trafficking in murine and amphibian models. Recently, powerful and cost-effective molecular tools and techniques for zebrafish have opened new opportunities to study photoreceptor IFT. Studies using zebrafish take advantage of its rapid embryogenesis to characterize the early events involved in photoreceptor ciliogenesis and OS assembly. In this overview, we describe phenotypes associated with knockdown strategies or genetic mutations of IFT components in zebrafish and detail a general experimental approach that has enabled us to study the function of the two anterograde IFT motors, KIF17 and kinesin II, in zebrafish cone photoreceptors. 2009 Elsevier Inc. All rights reserved.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 219-234 |
| Number of pages | 16 |
| Journal | Methods in Cell Biology |
| Volume | 93 |
| DOIs | |
| State | Published - 2009 |
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ASJC Scopus subject areas
- Cell Biology
Cite this
Analysis of IFT kinesins in developing zebrafish cone photoreceptor sensory cilia. / Insinna, Christine; Luby-Phelps, Katherine; Link, Brian A.; Besharse, Joseph C.
In: Methods in Cell Biology, Vol. 93, 2009, p. 219-234.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of IFT kinesins in developing zebrafish cone photoreceptor sensory cilia.
AU - Insinna, Christine
AU - Luby-Phelps, Katherine
AU - Link, Brian A.
AU - Besharse, Joseph C.
PY - 2009
Y1 - 2009
N2 - The photoreceptor outer segment (OS), a well-defined sensory cilium, provides an important context for the study of intraflagellar transport (IFT). The early phases of OS development involve successive events that are common to virtually all cilia. Additionally, intense protein trafficking occurs through the cilium and relies on IFT to maintain proper cellular morphology and optimize the photosensitive function. In the past decade, progress has been made in the characterization of photoreceptor OS trafficking in murine and amphibian models. Recently, powerful and cost-effective molecular tools and techniques for zebrafish have opened new opportunities to study photoreceptor IFT. Studies using zebrafish take advantage of its rapid embryogenesis to characterize the early events involved in photoreceptor ciliogenesis and OS assembly. In this overview, we describe phenotypes associated with knockdown strategies or genetic mutations of IFT components in zebrafish and detail a general experimental approach that has enabled us to study the function of the two anterograde IFT motors, KIF17 and kinesin II, in zebrafish cone photoreceptors. 2009 Elsevier Inc. All rights reserved.
AB - The photoreceptor outer segment (OS), a well-defined sensory cilium, provides an important context for the study of intraflagellar transport (IFT). The early phases of OS development involve successive events that are common to virtually all cilia. Additionally, intense protein trafficking occurs through the cilium and relies on IFT to maintain proper cellular morphology and optimize the photosensitive function. In the past decade, progress has been made in the characterization of photoreceptor OS trafficking in murine and amphibian models. Recently, powerful and cost-effective molecular tools and techniques for zebrafish have opened new opportunities to study photoreceptor IFT. Studies using zebrafish take advantage of its rapid embryogenesis to characterize the early events involved in photoreceptor ciliogenesis and OS assembly. In this overview, we describe phenotypes associated with knockdown strategies or genetic mutations of IFT components in zebrafish and detail a general experimental approach that has enabled us to study the function of the two anterograde IFT motors, KIF17 and kinesin II, in zebrafish cone photoreceptors. 2009 Elsevier Inc. All rights reserved.
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UR - http://www.scopus.com/inward/citedby.url?scp=77954674137&partnerID=8YFLogxK
U2 - 10.1016/S0091-679X(08)93012-0
DO - 10.1016/S0091-679X(08)93012-0
M3 - Article
C2 - 20409820
AN - SCOPUS:77954674137
VL - 93
SP - 219
EP - 234
JO - Methods in Cell Biology
JF - Methods in Cell Biology
SN - 0091-679X
ER -