Analysis of mechanisms associated with loss of infectivity of clonal populations of Borrelia burgdorferi B31MI

J. V. McDowell, Ying Sung Shian Ying Sung, M. Labandeira-Rey, J. T. Skare, R. T. Marconi

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Numerous studies have provided suggestive evidence that the loss of plasmids correlates with the loss of infectivity of the Lyme disease spirochetes. In this study we have further investigated this correlation. Clonal populations were obtained from the skin of a mouse infected for 3 months with a clonal population of Borrelia burgdorferi B31MI. The complete plasmid compositions of these populations were determined using a combination of PCR and Southern hybridization. The infectivities of clones differing in plasmid composition were tested using the C3H-HeJ murine model for Lyme disease. While several clones were found to be noninfectious, a correlation between the loss of a specific plasmid and loss of infectivity in the clones analyzed in this report was not observed. While it is clear from recent studies that the loss of some specific plasmids results in attenuated virulence, this study demonstrates that additional mechanisms also contribute to the loss of infectivity.

Original languageEnglish (US)
Pages (from-to)3670-3677
Number of pages8
JournalInfection and immunity
Volume69
Issue number6
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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