TY - JOUR
T1 - Analysis of posttranslational regulations in the Neurospora circadian clock
AU - Liu, Yi
N1 - Funding Information:
This work was supported by grants from the National Institutes of Health (GM062591 and GM068496) and Welch Foundation. The author is the Louise W. Kahn Scholar in Biomedical Research at University of Texas Southwestern Medical Center.
PY - 2005
Y1 - 2005
N2 - Posttranslational modification of circadian clock proteins by phosphorylation is an essential regulatory process in the control of eukaryotic circadian clocks. In the Neurospora circadian clock, the key clock protein FREQUENCY (FRQ) is progressively phosphorylated. The phosphorylation of FRQ is regulated by both kinases and phosphatases, and the phosphorylation is important for regulating FRQ stability and its function in the circadian negative feedback loop. The degradation of FRQ is mediated by the ubiquitin/proteasome pathway. This article discusses posttranslational regulations of the Neurospora clock and describes the methods used in the studies of FRQ phosphorylation, FRQ kinases and phosphatases, and FRQ degradation.
AB - Posttranslational modification of circadian clock proteins by phosphorylation is an essential regulatory process in the control of eukaryotic circadian clocks. In the Neurospora circadian clock, the key clock protein FREQUENCY (FRQ) is progressively phosphorylated. The phosphorylation of FRQ is regulated by both kinases and phosphatases, and the phosphorylation is important for regulating FRQ stability and its function in the circadian negative feedback loop. The degradation of FRQ is mediated by the ubiquitin/proteasome pathway. This article discusses posttranslational regulations of the Neurospora clock and describes the methods used in the studies of FRQ phosphorylation, FRQ kinases and phosphatases, and FRQ degradation.
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U2 - 10.1016/S0076-6879(05)93017-6
DO - 10.1016/S0076-6879(05)93017-6
M3 - Article
C2 - 15817300
AN - SCOPUS:16844385822
SN - 0076-6879
VL - 393
SP - 379
EP - 393
JO - Methods in Enzymology
JF - Methods in Enzymology
M1 - 17
ER -