Analysis of posttranslational regulations in the Neurospora circadian clock

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Posttranslational modification of circadian clock proteins by phosphorylation is an essential regulatory process in the control of eukaryotic circadian clocks. In the Neurospora circadian clock, the key clock protein FREQUENCY (FRQ) is progressively phosphorylated. The phosphorylation of FRQ is regulated by both kinases and phosphatases, and the phosphorylation is important for regulating FRQ stability and its function in the circadian negative feedback loop. The degradation of FRQ is mediated by the ubiquitin/proteasome pathway. This article discusses posttranslational regulations of the Neurospora clock and describes the methods used in the studies of FRQ phosphorylation, FRQ kinases and phosphatases, and FRQ degradation.

Original languageEnglish (US)
Article number17
Pages (from-to)379-393
Number of pages15
JournalMethods in Enzymology
Volume393
DOIs
StatePublished - 2005

Fingerprint

Neurospora
Circadian Clocks
Phosphorylation
Clocks
Phosphoric Monoester Hydrolases
Phosphotransferases
Proteasome Endopeptidase Complex
Post Translational Protein Processing
Ubiquitin
Degradation
Proteins
Feedback

ASJC Scopus subject areas

  • Biochemistry

Cite this

Analysis of posttranslational regulations in the Neurospora circadian clock. / Liu, Yi.

In: Methods in Enzymology, Vol. 393, 17, 2005, p. 379-393.

Research output: Contribution to journalArticle

@article{28fad81cbbae4dce84db8ca4bbc9c514,
title = "Analysis of posttranslational regulations in the Neurospora circadian clock",
abstract = "Posttranslational modification of circadian clock proteins by phosphorylation is an essential regulatory process in the control of eukaryotic circadian clocks. In the Neurospora circadian clock, the key clock protein FREQUENCY (FRQ) is progressively phosphorylated. The phosphorylation of FRQ is regulated by both kinases and phosphatases, and the phosphorylation is important for regulating FRQ stability and its function in the circadian negative feedback loop. The degradation of FRQ is mediated by the ubiquitin/proteasome pathway. This article discusses posttranslational regulations of the Neurospora clock and describes the methods used in the studies of FRQ phosphorylation, FRQ kinases and phosphatases, and FRQ degradation.",
author = "Yi Liu",
year = "2005",
doi = "10.1016/S0076-6879(05)93017-6",
language = "English (US)",
volume = "393",
pages = "379--393",
journal = "Methods in Enzymology",
issn = "0076-6879",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Analysis of posttranslational regulations in the Neurospora circadian clock

AU - Liu, Yi

PY - 2005

Y1 - 2005

N2 - Posttranslational modification of circadian clock proteins by phosphorylation is an essential regulatory process in the control of eukaryotic circadian clocks. In the Neurospora circadian clock, the key clock protein FREQUENCY (FRQ) is progressively phosphorylated. The phosphorylation of FRQ is regulated by both kinases and phosphatases, and the phosphorylation is important for regulating FRQ stability and its function in the circadian negative feedback loop. The degradation of FRQ is mediated by the ubiquitin/proteasome pathway. This article discusses posttranslational regulations of the Neurospora clock and describes the methods used in the studies of FRQ phosphorylation, FRQ kinases and phosphatases, and FRQ degradation.

AB - Posttranslational modification of circadian clock proteins by phosphorylation is an essential regulatory process in the control of eukaryotic circadian clocks. In the Neurospora circadian clock, the key clock protein FREQUENCY (FRQ) is progressively phosphorylated. The phosphorylation of FRQ is regulated by both kinases and phosphatases, and the phosphorylation is important for regulating FRQ stability and its function in the circadian negative feedback loop. The degradation of FRQ is mediated by the ubiquitin/proteasome pathway. This article discusses posttranslational regulations of the Neurospora clock and describes the methods used in the studies of FRQ phosphorylation, FRQ kinases and phosphatases, and FRQ degradation.

UR - http://www.scopus.com/inward/record.url?scp=16844385822&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16844385822&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(05)93017-6

DO - 10.1016/S0076-6879(05)93017-6

M3 - Article

C2 - 15817300

AN - SCOPUS:16844385822

VL - 393

SP - 379

EP - 393

JO - Methods in Enzymology

JF - Methods in Enzymology

SN - 0076-6879

M1 - 17

ER -