Analysis of radioactive and nonradioactive purine bases, nucleosides, and nucleotides by high-speed chromatography on a single column

Bohdan Bakay, Erkki Nissinen, Lawrence Sweetman

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The concentrations of radioactive and nonradioactive purine bases, purine nucleosides, purine mono-, di-, and trinucleotides in acid extracts of fibroblasts were determined by anion-exchange column chromatography. The concentrations of nonradioactive components were determined by computerized integration of the signal from a double-beam uv-detector. The radioactive metabolites were quantitated by high-efficiency, continuous liquid scintillation counting, employing a discrete sample transport system.

Original languageEnglish (US)
Pages (from-to)65-77
Number of pages13
JournalAnalytical biochemistry
Volume86
Issue number1
DOIs
StatePublished - May 1978
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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