Analysis of RNA-protein interactions by cell mixing

Sarah H. Stubbs, Nicholas K. Conrad

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

RNA-protein complexes are critical for almost all aspects of gene expression. Analysis of RNA-protein interactions can be complicated by the disruption of native complexes and the formation of new, reassorted complexes upon cell lysis. Before concluding that a specific RNA and protein interact in vivo, cell-mixing experiments can be performed to ensure that observed RNA-protein complexes are not formed after lysis of cells.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationProtein Part B
PublisherAcademic Press Inc.
Pages67-80
Number of pages14
ISBN (Print)9780124201200
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume539
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Stubbs, S. H., & Conrad, N. K. (2014). Analysis of RNA-protein interactions by cell mixing. In Laboratory Methods in Enzymology: Protein Part B (pp. 67-80). (Methods in Enzymology; Vol. 539). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-420120-0.00005-0