Analysis of RNA-protein interactions by cell mixing

Sarah H. Stubbs, Nicholas K. Conrad

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

RNA-protein complexes are critical for almost all aspects of gene expression. Analysis of RNA-protein interactions can be complicated by the disruption of native complexes and the formation of new, reassorted complexes upon cell lysis. Before concluding that a specific RNA and protein interact in vivo, cell-mixing experiments can be performed to ensure that observed RNA-protein complexes are not formed after lysis of cells.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages67-80
Number of pages14
Volume539
ISBN (Print)9780124201200
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume539
ISSN (Print)00766879
ISSN (Electronic)15577988

Fingerprint

Cell Communication
RNA
Proteins
Gene expression
Gene Expression
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Stubbs, S. H., & Conrad, N. K. (2014). Analysis of RNA-protein interactions by cell mixing. In Methods in Enzymology (Vol. 539, pp. 67-80). (Methods in Enzymology; Vol. 539). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-420120-0.00005-0

Analysis of RNA-protein interactions by cell mixing. / Stubbs, Sarah H.; Conrad, Nicholas K.

Methods in Enzymology. Vol. 539 Academic Press Inc., 2014. p. 67-80 (Methods in Enzymology; Vol. 539).

Research output: Chapter in Book/Report/Conference proceedingChapter

Stubbs, SH & Conrad, NK 2014, Analysis of RNA-protein interactions by cell mixing. in Methods in Enzymology. vol. 539, Methods in Enzymology, vol. 539, Academic Press Inc., pp. 67-80. https://doi.org/10.1016/B978-0-12-420120-0.00005-0
Stubbs SH, Conrad NK. Analysis of RNA-protein interactions by cell mixing. In Methods in Enzymology. Vol. 539. Academic Press Inc. 2014. p. 67-80. (Methods in Enzymology). https://doi.org/10.1016/B978-0-12-420120-0.00005-0
Stubbs, Sarah H. ; Conrad, Nicholas K. / Analysis of RNA-protein interactions by cell mixing. Methods in Enzymology. Vol. 539 Academic Press Inc., 2014. pp. 67-80 (Methods in Enzymology).
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