TY - JOUR
T1 - Analysis of telomere lengths in human corneal endothelial cells from donors of different ages
AU - Egan, C. A.
AU - Savre-Train, I.
AU - Boume, W. M.
AU - Shay, J. W.
PY - 1997
Y1 - 1997
N2 - Purpose. To investigate the telornere hypothesis of cellular aging as the mechanism for cell cycle arrest in normal human corneal endothelium. Methods. The corneal endothelium and epithelium from human corneas from donors aged 6 weeks, 13 months, and 16, 31, 40, 54, and 84 years were dissected and frozen at -70°C. Purified DNA, digested with the restriction enzyme, Hinfl, was run on 0.7% agarose gels, probed with radiolabelled (AATCCC)4, and exposed to a Phosphorlmager screen (Molecular Dynamics). The length of the peak terminal restriction fragment (TRF) signal was determined by densitometry using ImageQuant software. Results. The endothelial cells had TRF lengths ranging from 10.8-12.0 kbp (mean 11.57). Corneal epithelial samples showed variable TRF lengths (mean 10.41. range 7.9-11.5). Conclusion. Human corneal endothelial cells have long telomeres. Their limited replicative ability is not explained by critically short telomere lengths.
AB - Purpose. To investigate the telornere hypothesis of cellular aging as the mechanism for cell cycle arrest in normal human corneal endothelium. Methods. The corneal endothelium and epithelium from human corneas from donors aged 6 weeks, 13 months, and 16, 31, 40, 54, and 84 years were dissected and frozen at -70°C. Purified DNA, digested with the restriction enzyme, Hinfl, was run on 0.7% agarose gels, probed with radiolabelled (AATCCC)4, and exposed to a Phosphorlmager screen (Molecular Dynamics). The length of the peak terminal restriction fragment (TRF) signal was determined by densitometry using ImageQuant software. Results. The endothelial cells had TRF lengths ranging from 10.8-12.0 kbp (mean 11.57). Corneal epithelial samples showed variable TRF lengths (mean 10.41. range 7.9-11.5). Conclusion. Human corneal endothelial cells have long telomeres. Their limited replicative ability is not explained by critically short telomere lengths.
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M3 - Article
AN - SCOPUS:33749132786
SN - 0146-0404
VL - 38
SP - S400
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 4
ER -