Analysis of telomeres and telomerase.

Brittney Shea Herbert, Jerry W. Shay, Woodring E. Wright

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This unit describes techniques to analyze telomeric length and telomerase activity in human cells. Telomere length can be determined by a modification of Southern blotting in which the analysis of chromosome terminal restriction fragments (TRFs) provides the average lengths of all telomeres in a cell population. Telomerase activity can be measured in vitro by a sensitive and efficient polymerase chain reaction (PCR)-based detection method, also known as telomeric repeat amplification protocol (TRAP). These assays can be used to study the in vitro cellular effects of aging and cancer treatments on telomere biology and telomerase activity.

Original languageEnglish (US)
JournalCurrent protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]
VolumeChapter 18
StatePublished - Nov 2003

Fingerprint

Telomerase
Telomere
Cell Aging
Southern Blotting
Human Activities
Chromosomes
Polymerase Chain Reaction
Population
Neoplasms
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Analysis of telomeres and telomerase. / Herbert, Brittney Shea; Shay, Jerry W.; Wright, Woodring E.

In: Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.], Vol. Chapter 18, 11.2003.

Research output: Contribution to journalArticle

@article{6b5d78be04dd46dbb0fa67a88466af90,
title = "Analysis of telomeres and telomerase.",
abstract = "This unit describes techniques to analyze telomeric length and telomerase activity in human cells. Telomere length can be determined by a modification of Southern blotting in which the analysis of chromosome terminal restriction fragments (TRFs) provides the average lengths of all telomeres in a cell population. Telomerase activity can be measured in vitro by a sensitive and efficient polymerase chain reaction (PCR)-based detection method, also known as telomeric repeat amplification protocol (TRAP). These assays can be used to study the in vitro cellular effects of aging and cancer treatments on telomere biology and telomerase activity.",
author = "Herbert, {Brittney Shea} and Shay, {Jerry W.} and Wright, {Woodring E.}",
year = "2003",
month = "11",
language = "English (US)",
volume = "Chapter 18",
journal = "Current Protocols in Cell Biology",
issn = "1934-2500",
publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Analysis of telomeres and telomerase.

AU - Herbert, Brittney Shea

AU - Shay, Jerry W.

AU - Wright, Woodring E.

PY - 2003/11

Y1 - 2003/11

N2 - This unit describes techniques to analyze telomeric length and telomerase activity in human cells. Telomere length can be determined by a modification of Southern blotting in which the analysis of chromosome terminal restriction fragments (TRFs) provides the average lengths of all telomeres in a cell population. Telomerase activity can be measured in vitro by a sensitive and efficient polymerase chain reaction (PCR)-based detection method, also known as telomeric repeat amplification protocol (TRAP). These assays can be used to study the in vitro cellular effects of aging and cancer treatments on telomere biology and telomerase activity.

AB - This unit describes techniques to analyze telomeric length and telomerase activity in human cells. Telomere length can be determined by a modification of Southern blotting in which the analysis of chromosome terminal restriction fragments (TRFs) provides the average lengths of all telomeres in a cell population. Telomerase activity can be measured in vitro by a sensitive and efficient polymerase chain reaction (PCR)-based detection method, also known as telomeric repeat amplification protocol (TRAP). These assays can be used to study the in vitro cellular effects of aging and cancer treatments on telomere biology and telomerase activity.

UR - http://www.scopus.com/inward/record.url?scp=39049163472&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39049163472&partnerID=8YFLogxK

M3 - Article

C2 - 18228424

AN - SCOPUS:39049163472

VL - Chapter 18

JO - Current Protocols in Cell Biology

JF - Current Protocols in Cell Biology

SN - 1934-2500

ER -