Analysis of the fully folded enzyme NodST inhibitor and substrate complexes by ESI-FTICR mass spectrometry

Yonghao Yu, Gary Kruppa, Na Pi, Ulla Andersen, Julie A. Leary

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fully folded enzyme NodST inhibitor and substrate complexes were analyzed using electrospray ionization (ESI)-FTICR Mass spectrometry. The values of K M (PAPS) and K i (PAP) were determined using solution phase kinetics. It was assumed that the high capillary exit voltage and higher pressure in the capillary skimmer region resulted in the ions being dragged through a viscous gas without being accelerated to higher kinetic energy. The K S/K i values determined from gas phase was found to be similar to the one obtained from solution phase, which suggested that the noncovalent complexes in the gas phase accurately reflect the solution phase equilibrium.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages409-410
Number of pages2
StatePublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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