Abstract
Fully folded enzyme NodST inhibitor and substrate complexes were analyzed using electrospray ionization (ESI)-FTICR Mass spectrometry. The values of K M (PAPS) and K i (PAP) were determined using solution phase kinetics. It was assumed that the high capillary exit voltage and higher pressure in the capillary skimmer region resulted in the ions being dragged through a viscous gas without being accelerated to higher kinetic energy. The K S/K i values determined from gas phase was found to be similar to the one obtained from solution phase, which suggested that the noncovalent complexes in the gas phase accurately reflect the solution phase equilibrium.
Original language | English (US) |
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Title of host publication | Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics |
Pages | 409-410 |
Number of pages | 2 |
State | Published - 2002 |
Event | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002 |
Other
Other | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
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Country/Territory | United States |
City | Orlando, FL |
Period | 6/2/02 → 6/6/02 |
ASJC Scopus subject areas
- Spectroscopy