Analysis of the guinea-pig estrogen-regulated gec1/GABARAPL1 gene promoter and identification of a functional ERE in the first exon

Sandrine Vernier-Magnin, Christophe Nemos, Virginie Mansuy, Fabrice Tolle, Laure Guichard, Régis Delage-Mourroux, Michèle Jouvenot, Annick Fraichard

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The gec1/GABARAPL1 (GABAA-receptor-associated protein like-1) gene has been identified as an early estrogen-regulated gene in guinea-pig cultured endometrial glandular epithelial cells (GEC). Guinea-pig and human gec1/GABARAPL1 proteins share 87% identity with GABARAP, which acts as a protein linker between microtubules and the GABAA receptor. To investigate the molecular mechanisms regulating gec1/GABARAPL1 gene expression, the 1.5-kbp region upstream of the translation initiation codon of the guinea-pig gec1/GABARAPL1 gene was cloned. A 300-bp fragment encompassing a pyrimidine-rich initiator element (INR) and the transcription start site (+1) was sufficient to initiate transcription. Transfection and gel shift experiments showed that a sequence located at +36/+50 in the first exon permitted induction of expression of this gene by estradiol acting via ERα. This sequence (GGGTCAACGTGACGT) differs only by one base pair from the consensus estrogen response element ERE (GGGTCAACGTGACCT). It can be concluded that the ERE located in the first exon encoding the 5′-untranslated region is sufficient for E2 activation of gec1/GABARAPL1 transcription.

Original languageEnglish (US)
Pages (from-to)23-31
Number of pages9
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1731
Issue number1
DOIs
StatePublished - Oct 15 2005

Keywords

  • 17β-estradiol (E)
  • ERE (estrogen response element)
  • Early estrogen-regulated gene
  • Estrogen receptors (ERα and ERβ)
  • INR (initiator element)
  • gec1/GABARAPL1

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Fingerprint Dive into the research topics of 'Analysis of the guinea-pig estrogen-regulated gec1/GABARAPL1 gene promoter and identification of a functional ERE in the first exon'. Together they form a unique fingerprint.

  • Cite this