TY - JOUR
T1 - Analysis of the major patterns of B cell gene expression changes in response to short-term stimulation with 33 single ligands
AU - Zhu, Xiaocui
AU - Hart, Rebecca
AU - Chang, Mi Sook
AU - Kim, Jong Woo
AU - Lee, Sun Young
AU - Cao, Yun Anna
AU - Mock, Dennis
AU - Ke, Eugene
AU - Saunders, Brian
AU - Alexander, Angela
AU - Grossoehme, Joella
AU - Lin, Keng Mean
AU - Yan, Zhen
AU - Hsueh, Robert
AU - Lee, Jamie
AU - Scheuermann, Richard H.
AU - Fruman, David A.
AU - Seaman, William
AU - Subramaniam, Shankar
AU - Sternweis, Paul
AU - Simon, Melvin I.
AU - Choi, Sangdun
PY - 2004/12/15
Y1 - 2004/12/15
N2 - We examined the major patterns of changes in gene expression in mouse splenic B cells in response to stimulation with 33 single ligands for 0.5, 1, 2, and 4 h. We found that ligands known to directly induce or costimulate proliferation, namely, anti-IgM (anti-Ig), anti-CD40 (CD40L), LPS, and, to a lesser extent, IL-4 and CpG-oligodeoxynucleotide (CpG), induced significant expression changes in a large number of genes. The remaining 28 single ligands produced changes in relatively few genes, even though they elicited measurable elevations in intracellular Ca2+ and cAMP concentration and/or protein phosphorylation, including cytokines, chemokines, and other ligands that interact with G protein-coupled receptors. A detailed comparison of gene expression responses to anti-Ig, CD40L, LPS, IL-4, and CpG indicates that while many genes had similar temporal patterns of change in expression in response to these ligands, subsets of genes showed unique expression patterns in response to IL-4, anti-Ig, and CD40L.
AB - We examined the major patterns of changes in gene expression in mouse splenic B cells in response to stimulation with 33 single ligands for 0.5, 1, 2, and 4 h. We found that ligands known to directly induce or costimulate proliferation, namely, anti-IgM (anti-Ig), anti-CD40 (CD40L), LPS, and, to a lesser extent, IL-4 and CpG-oligodeoxynucleotide (CpG), induced significant expression changes in a large number of genes. The remaining 28 single ligands produced changes in relatively few genes, even though they elicited measurable elevations in intracellular Ca2+ and cAMP concentration and/or protein phosphorylation, including cytokines, chemokines, and other ligands that interact with G protein-coupled receptors. A detailed comparison of gene expression responses to anti-Ig, CD40L, LPS, IL-4, and CpG indicates that while many genes had similar temporal patterns of change in expression in response to these ligands, subsets of genes showed unique expression patterns in response to IL-4, anti-Ig, and CD40L.
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U2 - 10.4049/jimmunol.173.12.7141
DO - 10.4049/jimmunol.173.12.7141
M3 - Article
C2 - 15585835
AN - SCOPUS:10344227714
SN - 0022-1767
VL - 173
SP - 7141
EP - 7149
JO - Journal of Immunology
JF - Journal of Immunology
IS - 12
ER -