Analysis of the major patterns of B cell gene expression changes in response to short-term stimulation with 33 single ligands

Xiaocui Zhu, Rebecca Hart, Mi Sook Chang, Jong Woo Kim, Sun Young Lee, Yun Anna Cao, Dennis Mock, Eugene Ke, Brian Saunders, Angela Alexander, Joella Grossoehme, Keng Mean Lin, Zhen Yan, Robert Hsueh, Jamie Lee, Richard H. Scheuermann, David A. Fruman, William Seaman, Shankar Subramaniam, Paul SternweisMelvin I. Simon, Sangdun Choi

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40 Scopus citations

Abstract

We examined the major patterns of changes in gene expression in mouse splenic B cells in response to stimulation with 33 single ligands for 0.5, 1, 2, and 4 h. We found that ligands known to directly induce or costimulate proliferation, namely, anti-IgM (anti-Ig), anti-CD40 (CD40L), LPS, and, to a lesser extent, IL-4 and CpG-oligodeoxynucleotide (CpG), induced significant expression changes in a large number of genes. The remaining 28 single ligands produced changes in relatively few genes, even though they elicited measurable elevations in intracellular Ca2+ and cAMP concentration and/or protein phosphorylation, including cytokines, chemokines, and other ligands that interact with G protein-coupled receptors. A detailed comparison of gene expression responses to anti-Ig, CD40L, LPS, IL-4, and CpG indicates that while many genes had similar temporal patterns of change in expression in response to these ligands, subsets of genes showed unique expression patterns in response to IL-4, anti-Ig, and CD40L.

Original languageEnglish (US)
Pages (from-to)7141-7149
Number of pages9
JournalJournal of Immunology
Volume173
Issue number12
DOIs
StatePublished - Dec 15 2004

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Zhu, X., Hart, R., Chang, M. S., Kim, J. W., Lee, S. Y., Cao, Y. A., Mock, D., Ke, E., Saunders, B., Alexander, A., Grossoehme, J., Lin, K. M., Yan, Z., Hsueh, R., Lee, J., Scheuermann, R. H., Fruman, D. A., Seaman, W., Subramaniam, S., ... Choi, S. (2004). Analysis of the major patterns of B cell gene expression changes in response to short-term stimulation with 33 single ligands. Journal of Immunology, 173(12), 7141-7149. https://doi.org/10.4049/jimmunol.173.12.7141