Analysis of the tendon cell fate using Scleraxis, a specific marker for tendons and ligaments

R. Schweitzer, J. H. Chyung, L. C. Murtaugh, A. E. Brent, V. Rosen, E. N. Olson, A. Lassar, C. J. Tabin

Research output: Contribution to journalArticle

511 Citations (Scopus)

Abstract

Little is known about the genesis and patterning of tendons and other connective tissues, mostly owing to the absence of early markers. We have found that Scleraxis, a bHLH transcription factor, is a highly specific marker for all the connective tissues that mediate attachment of muscle to bone in chick and mouse, including the limb tendons, and show that early scleraxis expression marks the progenitor cell populations for these tissues. In the early limb bud, the tendon progenitor population is found in the superficial proximomedial mesenchyme. Using the scleraxis gene as a marker we show that these progenitors are induced by ectodermal signals and restricted by bone morphogenetic protein (BMP) signaling within the mesenchyme. Application of Noggin protein antagonizes this endogenous BMP activity and induces ectopic scleraxis expression. However, the presence of excess tendon progenitors does not lead to the production of additional or longer tendons, indicating that additional signals are required for the final formation of a tendon. Finally, we show that the endogenous expression of noggin within the condensing digit cartilage contributes to the induction of distal tendons.

Original languageEnglish (US)
Pages (from-to)3855-3866
Number of pages12
JournalDevelopment
Volume128
Issue number19
StatePublished - 2001

Fingerprint

Ligaments
Tendons
Bone Morphogenetic Proteins
Mesoderm
Connective Tissue
Basic Helix-Loop-Helix Transcription Factors
Limb Buds
Population
Cartilage
Stem Cells
Extremities
Bone and Bones
Muscles
Genes

Keywords

  • Cell fate
  • Chick
  • Mouse
  • Musculoskeletal system
  • Scleraxis
  • Tendon

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Schweitzer, R., Chyung, J. H., Murtaugh, L. C., Brent, A. E., Rosen, V., Olson, E. N., ... Tabin, C. J. (2001). Analysis of the tendon cell fate using Scleraxis, a specific marker for tendons and ligaments. Development, 128(19), 3855-3866.

Analysis of the tendon cell fate using Scleraxis, a specific marker for tendons and ligaments. / Schweitzer, R.; Chyung, J. H.; Murtaugh, L. C.; Brent, A. E.; Rosen, V.; Olson, E. N.; Lassar, A.; Tabin, C. J.

In: Development, Vol. 128, No. 19, 2001, p. 3855-3866.

Research output: Contribution to journalArticle

Schweitzer, R, Chyung, JH, Murtaugh, LC, Brent, AE, Rosen, V, Olson, EN, Lassar, A & Tabin, CJ 2001, 'Analysis of the tendon cell fate using Scleraxis, a specific marker for tendons and ligaments', Development, vol. 128, no. 19, pp. 3855-3866.
Schweitzer R, Chyung JH, Murtaugh LC, Brent AE, Rosen V, Olson EN et al. Analysis of the tendon cell fate using Scleraxis, a specific marker for tendons and ligaments. Development. 2001;128(19):3855-3866.
Schweitzer, R. ; Chyung, J. H. ; Murtaugh, L. C. ; Brent, A. E. ; Rosen, V. ; Olson, E. N. ; Lassar, A. ; Tabin, C. J. / Analysis of the tendon cell fate using Scleraxis, a specific marker for tendons and ligaments. In: Development. 2001 ; Vol. 128, No. 19. pp. 3855-3866.
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