Analysis of the very large G-protein coupled receptor gene (Vlgr1/Mass1/USH2C) in zebrafish

Yann Gibert, D. Randy McMillan, Kathleen Kayes-Wandover, Axel Meyer, Gerrit Begemann, Perrin C. White

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Very Large G-protein coupled Receptor-1 (VLGR1/Mass1/USH2C) is the largest known cell surface protein in vertebrates. Mutations in VLGR1 are associated with audiogenic epilepsy in mice and Usher syndrome (sensorineural deafness and retinitis pigmentosa) in humans. We characterized the zebrafish VLGR1 gene (vlgr1). It is 51% identical to human VLGR1 in amino acid sequence, but is 64% identical in the 7-transmembrane and cytoplasmic domains. It is 6199 amino acids in size and is encoded by a 19.2 kb mRNA. All introns correspond in location and phase to those of the human and mouse genes. In situ hybridization studies of zebrafish embryos demonstrate vlgr1 expression in the developing central nervous system, particularly in the hypothalamus, epiphysis and in the rhombic lips. Expression in the eye is associated with the optic nerve. Further studies using zebrafish may help ascertain the role of Vlgr1 in neural development.

Original languageEnglish (US)
Pages (from-to)200-206
Number of pages7
JournalGene
Volume353
Issue number2
DOIs
StatePublished - Jul 4 2005

Fingerprint

Zebrafish
G-Protein-Coupled Receptors
Usher Syndromes
Reflex Epilepsy
Genes
Epiphyses
Retinitis Pigmentosa
Deafness
Optic Nerve
Lip
Introns
Hypothalamus
In Situ Hybridization
Vertebrates
Amino Acid Sequence
Membrane Proteins
Embryonic Structures
Central Nervous System
Amino Acids
Messenger RNA

Keywords

  • Diencephalon
  • In situ hybridization
  • Optic nerve
  • Usher syndrome
  • Zebrafish

ASJC Scopus subject areas

  • Genetics

Cite this

Gibert, Y., McMillan, D. R., Kayes-Wandover, K., Meyer, A., Begemann, G., & White, P. C. (2005). Analysis of the very large G-protein coupled receptor gene (Vlgr1/Mass1/USH2C) in zebrafish. Gene, 353(2), 200-206. https://doi.org/10.1016/j.gene.2005.05.015

Analysis of the very large G-protein coupled receptor gene (Vlgr1/Mass1/USH2C) in zebrafish. / Gibert, Yann; McMillan, D. Randy; Kayes-Wandover, Kathleen; Meyer, Axel; Begemann, Gerrit; White, Perrin C.

In: Gene, Vol. 353, No. 2, 04.07.2005, p. 200-206.

Research output: Contribution to journalArticle

Gibert, Y, McMillan, DR, Kayes-Wandover, K, Meyer, A, Begemann, G & White, PC 2005, 'Analysis of the very large G-protein coupled receptor gene (Vlgr1/Mass1/USH2C) in zebrafish', Gene, vol. 353, no. 2, pp. 200-206. https://doi.org/10.1016/j.gene.2005.05.015
Gibert, Yann ; McMillan, D. Randy ; Kayes-Wandover, Kathleen ; Meyer, Axel ; Begemann, Gerrit ; White, Perrin C. / Analysis of the very large G-protein coupled receptor gene (Vlgr1/Mass1/USH2C) in zebrafish. In: Gene. 2005 ; Vol. 353, No. 2. pp. 200-206.
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