Analysis of transcriptional regulatory regions in vivo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Understanding the transcriptional regulation of development and tissue- specific gene expression is a central goal of modern biology. Although the analysis of gene transcription in transfected cultured cells has been essential in establishing many key aspects of this gene control, only analysis in animals can determine developmental timing and cell-specificity of expression within a complex organ and in all the tissues of an animal. The advent of transgenesis made in vivo studies possible. A summary of the in vivo regulatory properties of the pancreas-specific transcriptional enhancer of the rat elastase 1 gene (ELA1) and the role individual elements in this enhancer play in directing high level, cell-specific transcription illustrates the nature, revelations and limitations of transgenic analysis.

Original languageEnglish (US)
Pages (from-to)983-994
Number of pages12
JournalInternational Journal of Developmental Biology
Volume42
Issue number7
StatePublished - 1998

Fingerprint

Nucleic Acid Regulatory Sequences
Genes
Gene Transfer Techniques
Pancreatic Elastase
Pancreas
Cultured Cells
Gene Expression

Keywords

  • Elastase
  • Pancreas
  • Promoter
  • Transcriptional enhancer
  • Transgenic

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Analysis of transcriptional regulatory regions in vivo. / Macdonald, Raymond J.; Swift, Galvin H.

In: International Journal of Developmental Biology, Vol. 42, No. 7, 1998, p. 983-994.

Research output: Contribution to journalArticle

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