@article{5ee88db970314bd99d6d13e9aa4e8e0d,
title = "Analysis of tumor metabolism reveals mitochondrial glucose oxidation in genetically diverse human glioblastomas in the mouse brain in vivo",
abstract = "Dysregulated metabolism is a hallmark of cancer cell lines, but little is known about the fate of glucose and other nutrients in tumors growing in their native microenvironment. To study tumor metabolism in vivo, we used an orthotopic mouse model of primary human glioblastoma (GBM). We infused 13C-labeled nutrients into mice bearing three independent GBM lines, each with a distinct set of mutations. All three lines displayed glycolysis, as expected for aggressive tumors. They also displayed unexpected metabolic complexity, oxidizing glucose via pyruvate dehydrogenase and the citric acid cycle, and using glucose to supply anaplerosis and other biosynthetic activities. Comparing the tumors to surrounding brain revealed obvious metabolic differences, notably the accumulation of a large glutamine pool within the tumors. Many of these same activities were conserved in cells cultured ex vivo from the tumors. Thus GBM cells utilize mitochondrial glucose oxidation during aggressive tumor growth in vivo.",
author = "Isaac Marin-Valencia and Chendong Yang and Tomoyuki Mashimo and Steve Cho and Hyeonman Baek and Yang, {Xiao Li} and Rajagopalan, {Kartik N.} and Melissa Maddie and Vamsidhara Vemireddy and Zhenze Zhao and Ling Cai and Levi Good and Tu, {Benjamin P.} and Hatanpaa, {Kimmo J.} and Mickey, {Bruce E.} and Mat{\'e}s, {Jos{\'e} M.} and Pascual, {Juan M.} and Maher, {Elizabeth A.} and Malloy, {Craig R.} and DeBerardinis, {Ralph J.} and Bachoo, {Robert M.}",
note = "Funding Information: Changho Choi, Mark Jeffrey, and members of the DeBerardinis and Bachoo laboratories critically evaluated the data. Carolina Cardona purified the glutaminase antibody. Osamu Togao, Koji Sagiyama, and Masaya Takahashi performed the MRI scans and Xiankai Sun performed the PET scans. The authors were supported by NIH grants RR02584 and RC1NS0760675. In addition, I.M.V. was supported by the Fundaci{\'o}n Caja Madrid and the Billingsley Fund. R.J.D. was supported by grants from the NIH (DK072565 and CA157996), CPRIT (HIRP100437 and RP101243), Robert A. Welch Foundation (I-1733), and Damon Runyon Cancer Research Foundation. J.M.M. is supported by grants from Ministerio de Ciencia y Tecnolog{\'i}a of Spain (SAF2010-17573) and Junta de Andaluc{\'i}a (CVI-6656). ",
year = "2012",
month = jun,
day = "6",
doi = "10.1016/j.cmet.2012.05.001",
language = "English (US)",
volume = "15",
pages = "827--837",
journal = "Cell Metabolism",
issn = "1550-4131",
publisher = "Cell Press",
number = "6",
}