Analyzing autophagy in zebrafish

Congcong He, Daniel J. Klionsky

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

The transparency, external development and simple drug administration of zebrafish embryos makes them a useful model for studying autophagy during embryonic development in vivo. Cloning of zebrafish lc3 and generation of a transgenic GFP-Lc3 fish line provide excellent tools to monitor autophagy in this organism.1 This protocol discusses several convenient autophagy assays in zebrafish, including immunoblotting of Lc3 lipidation, microscopy imaging of GFP-Lc3 and lysosomal staining.

Original languageEnglish (US)
Pages (from-to)642-644
Number of pages3
JournalAutophagy
Volume6
Issue number5
DOIs
StatePublished - Jul 1 2010

Keywords

  • Lysosome
  • Protein degradation
  • Protein targeting
  • Stress
  • Vacuole

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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