Analyzing autophagy in zebrafish

Congcong He, Daniel J. Klionsky

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The transparency, external development and simple drug administration of zebrafish embryos makes them a useful model for studying autophagy during embryonic development in vivo. Cloning of zebrafish lc3 and generation of a transgenic GFP-Lc3 fish line provide excellent tools to monitor autophagy in this organism.1 This protocol discusses several convenient autophagy assays in zebrafish, including immunoblotting of Lc3 lipidation, microscopy imaging of GFP-Lc3 and lysosomal staining.

Original languageEnglish (US)
Pages (from-to)642-644
Number of pages3
JournalAutophagy
Volume6
Issue number5
DOIs
StatePublished - Jul 1 2010

Fingerprint

Autophagy
Zebrafish
Immunoblotting
Embryonic Development
Organism Cloning
Microscopy
Fishes
Embryonic Structures
Staining and Labeling
Pharmaceutical Preparations

Keywords

  • Lysosome
  • Protein degradation
  • Protein targeting
  • Stress
  • Vacuole

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

He, C., & Klionsky, D. J. (2010). Analyzing autophagy in zebrafish. Autophagy, 6(5), 642-644. https://doi.org/10.4161/auto.6.5.12092

Analyzing autophagy in zebrafish. / He, Congcong; Klionsky, Daniel J.

In: Autophagy, Vol. 6, No. 5, 01.07.2010, p. 642-644.

Research output: Contribution to journalArticle

He, C & Klionsky, DJ 2010, 'Analyzing autophagy in zebrafish', Autophagy, vol. 6, no. 5, pp. 642-644. https://doi.org/10.4161/auto.6.5.12092
He, Congcong ; Klionsky, Daniel J. / Analyzing autophagy in zebrafish. In: Autophagy. 2010 ; Vol. 6, No. 5. pp. 642-644.
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