Abstract
The transparency, external development and simple drug administration of zebrafish embryos makes them a useful model for studying autophagy during embryonic development in vivo. Cloning of zebrafish lc3 and generation of a transgenic GFP-Lc3 fish line provide excellent tools to monitor autophagy in this organism.1 This protocol discusses several convenient autophagy assays in zebrafish, including immunoblotting of Lc3 lipidation, microscopy imaging of GFP-Lc3 and lysosomal staining.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 642-644 |
| Number of pages | 3 |
| Journal | Autophagy |
| Volume | 6 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jul 1 2010 |
Keywords
- Lysosome
- Protein degradation
- Protein targeting
- Stress
- Vacuole
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology