TY - JOUR
T1 - Analyzing tumor metabolism in vivo
AU - Faubert, Brandon
AU - Deberardinis, Ralph J.
N1 - Funding Information:
We thank the members of the DeBerardinis laboratory for their helpful comments on this review. R.J.D. is supported by grants from the US National Institutes of Health (CA157996), the Cancer Prevention and Research Institute of Texas (RP160089), and the V Foundation for Cancer Research. B.F. is supported by the Canadian Institutes of Health Research (MFE-140911).
Publisher Copyright:
© 2017 by Annual Reviews.All rights reserved.
PY - 2017
Y1 - 2017
N2 - Altered metabolism is a clinically actionable hallmark of cancer. Some reprogrammed activities in cancer cells have predictive value and others are associated with therapeutic liabilities. Recent years have brought increased exploration of metabolism in intact tumors, complementing a large literature on cancer cell lines. We review the expanding tool kit available for studying the metabolic features of tumors in vivo. These techniques include metabolomics, positron emission tomography, magnetic resonance spectroscopy, and multiparametric magnetic resonance imaging, and they vary according to their invasiveness and breadth of metabolic assessment. Special attention is given to the emerging role of intraoperative infusions of stable, isotope-labeled nutrients, which have provided the first view of true metabolic flux in human tumors. These studies also demonstrate markedly different metabolic phenotypes from those observed in culture, indicating the potential for this approach to provide a disease-relevant view of cancer metabolism and to nominate new therapeutic targets from reprogrammed pathways.
AB - Altered metabolism is a clinically actionable hallmark of cancer. Some reprogrammed activities in cancer cells have predictive value and others are associated with therapeutic liabilities. Recent years have brought increased exploration of metabolism in intact tumors, complementing a large literature on cancer cell lines. We review the expanding tool kit available for studying the metabolic features of tumors in vivo. These techniques include metabolomics, positron emission tomography, magnetic resonance spectroscopy, and multiparametric magnetic resonance imaging, and they vary according to their invasiveness and breadth of metabolic assessment. Special attention is given to the emerging role of intraoperative infusions of stable, isotope-labeled nutrients, which have provided the first view of true metabolic flux in human tumors. These studies also demonstrate markedly different metabolic phenotypes from those observed in culture, indicating the potential for this approach to provide a disease-relevant view of cancer metabolism and to nominate new therapeutic targets from reprogrammed pathways.
KW - Cancer metabolism
KW - Magnetic resonance imaging
KW - Mass spectrometry
KW - Metabolic flux analysis
KW - Metabolomics
KW - Positron emission tomography
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U2 - 10.1146/annurev-cancerbio-050216-121954
DO - 10.1146/annurev-cancerbio-050216-121954
M3 - Article
AN - SCOPUS:85030558263
SN - 2472-3428
VL - 1
SP - 99
EP - 117
JO - Annual Review of Cancer Biology
JF - Annual Review of Cancer Biology
ER -