Androgen regulation of cell adhesion molecule gene expression in rat prostate during organ degeneration: C-CAM belongs to a class of androgen-repressed genes associated with enriched stem/amplifying cell population after prolonged castration

Jer Tsong Hsieh, Sue Hwa Lin

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Abstract

Androgen is required for maintaining the differentiated state of prostatic epithelium, and that cell adhesion molecules play active roles in controlling the development of epithelial cells. However, little is known about the regulation of cell adhesion molecules during prostate development. In this study, we demonstrated that the expression of an epithelial cell adhesion molecule, C-CAM, in rat ventral prostatic epithelium are repressed by androgen. A similar regulatory pattern was also observed in the seminal vesicle but not in other androgen-dependent organs (the coagulating gland and the dorsolateral prostate), or other organs (the liver and kidney). These observations suggest that regulation of C-CAM expression by androgen is tissue-specific. Unlike the other androgen-repressed genes associated with apoptosis, which have transient expression patterns, the elevated level of C-CAM in the ventral prostate persisted for more than 30 days postcastration, suggesting that C-CAM belongs to a class of androgen-repressed genes which are not associated with apoptosis. Immunohistochemical study detected C-CAM on the apical surface of secretory epithelium in control rats; castration resulted in an altered localization of C-CAM expression from columnar epithelium to cuboidal basal epithelium. Taken together, the up-regulation and altered expression pattern of C-CAM in ventral prostatic epithelium by androgen deprivation may be associated with the enriched epithelial stem/amplifying cell population in degenerated ventral prostate. These observations suggest that C-CAM may play an active role in the process of prostatic epithelial differentiation.

Original languageEnglish (US)
Pages (from-to)3711-3716
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number5
StatePublished - Feb 4 1994

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Castration
Cell Adhesion Molecules
Computer aided manufacturing
Stem cells
Gene expression
Androgens
Rats
Prostate
Stem Cells
Genes
Epithelium
Gene Expression
Population
Epithelial Cells
Apoptosis
Rat control
Seminal Vesicles
Liver
Up-Regulation
Kidney

ASJC Scopus subject areas

  • Biochemistry

Cite this

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title = "Androgen regulation of cell adhesion molecule gene expression in rat prostate during organ degeneration: C-CAM belongs to a class of androgen-repressed genes associated with enriched stem/amplifying cell population after prolonged castration",
abstract = "Androgen is required for maintaining the differentiated state of prostatic epithelium, and that cell adhesion molecules play active roles in controlling the development of epithelial cells. However, little is known about the regulation of cell adhesion molecules during prostate development. In this study, we demonstrated that the expression of an epithelial cell adhesion molecule, C-CAM, in rat ventral prostatic epithelium are repressed by androgen. A similar regulatory pattern was also observed in the seminal vesicle but not in other androgen-dependent organs (the coagulating gland and the dorsolateral prostate), or other organs (the liver and kidney). These observations suggest that regulation of C-CAM expression by androgen is tissue-specific. Unlike the other androgen-repressed genes associated with apoptosis, which have transient expression patterns, the elevated level of C-CAM in the ventral prostate persisted for more than 30 days postcastration, suggesting that C-CAM belongs to a class of androgen-repressed genes which are not associated with apoptosis. Immunohistochemical study detected C-CAM on the apical surface of secretory epithelium in control rats; castration resulted in an altered localization of C-CAM expression from columnar epithelium to cuboidal basal epithelium. Taken together, the up-regulation and altered expression pattern of C-CAM in ventral prostatic epithelium by androgen deprivation may be associated with the enriched epithelial stem/amplifying cell population in degenerated ventral prostate. These observations suggest that C-CAM may play an active role in the process of prostatic epithelial differentiation.",
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T1 - Androgen regulation of cell adhesion molecule gene expression in rat prostate during organ degeneration

T2 - C-CAM belongs to a class of androgen-repressed genes associated with enriched stem/amplifying cell population after prolonged castration

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AU - Lin, Sue Hwa

PY - 1994/2/4

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