TY - JOUR
T1 - Androgen repression of cytokeratin gene expression during rat prostate differentiation
T2 - evidence for an epithelial stem cell-associated marker.
AU - Wang, X.
AU - Hsieh, J. T.
PY - 1994/12
Y1 - 1994/12
N2 - Cytokeratin (CK) 8 mRNA expression in developing and degenerating rat prostate was studied using in situ hybridization with an antisense RNA-probe. It was found that: 1) the CK 8 antisense probe was accumulated only within prostatic epithelial cells; 2) after castration, CK 8 mRNA signals in ventral prostate (VP) sections were significantly increased, and elevated CK 8 mRNA expression persisted even long after prostate involution was complete; and 3) during prostate development, the strongest CK 8 mRNA staining was found in the early neonatal prostatic epithelia which were composed mainly of prostatic stem cells. Thereafter, a shift of CK 8 mRNA staining to peripheral regions and decreased overall CK 8 mRNA levels were noted. These data indicate that excessive expression of CK 8 mRNA is a characteristic of prostatic stem cells, and CK molecules are excellent markers for determining the hierarchical pathway of cell differentiation in prostate epithelium.
AB - Cytokeratin (CK) 8 mRNA expression in developing and degenerating rat prostate was studied using in situ hybridization with an antisense RNA-probe. It was found that: 1) the CK 8 antisense probe was accumulated only within prostatic epithelial cells; 2) after castration, CK 8 mRNA signals in ventral prostate (VP) sections were significantly increased, and elevated CK 8 mRNA expression persisted even long after prostate involution was complete; and 3) during prostate development, the strongest CK 8 mRNA staining was found in the early neonatal prostatic epithelia which were composed mainly of prostatic stem cells. Thereafter, a shift of CK 8 mRNA staining to peripheral regions and decreased overall CK 8 mRNA levels were noted. These data indicate that excessive expression of CK 8 mRNA is a characteristic of prostatic stem cells, and CK molecules are excellent markers for determining the hierarchical pathway of cell differentiation in prostate epithelium.
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M3 - Article
C2 - 7536487
AN - SCOPUS:0028726297
SN - 1001-9294
VL - 9
SP - 237
EP - 241
JO - Chinese medical sciences journal = Chung-kuo i hsüeh k'o hsüeh tsa chih / Chinese Academy of Medical Sciences
JF - Chinese medical sciences journal = Chung-kuo i hsüeh k'o hsüeh tsa chih / Chinese Academy of Medical Sciences
IS - 4
ER -