Androgen resistance due to decreased amounts of androgen receptor: A reinvestigation

Placido B. Grino, Jim Griffin III, Jean D. Wilson

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

To provide insight into the pathogenesis of the androgen resistance in a previously described family with X-linked Reifenstein syndrome, we have systematically assessed a variety of parameters of androgen receptor function in fibroblasts cultured from scrotal skin biopsies. We assessed the amount of high affinity binding, the affinity of ligand binding to the receptor, the upregulation of androgen receptor levels by androgen, the stability of ligand binding in intact fibroblasts at high temperature, the dissociation of ligand from the receptor, the intranuclear localization and salt elution profiles of ligand-receptor complexes, and the ultracentrifugation characteristics of the ligand-receptor complexes. Since the only parameter found to be abnormal is a decreased amount of receptor, we conclude that the underlying mutation in this family influences the amount rather than the structure of the androgen receptor protein.

Original languageEnglish (US)
Pages (from-to)647-654
Number of pages8
JournalJournal of Steroid Biochemistry
Volume35
Issue number6
DOIs
StatePublished - May 1990

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ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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