Angiopoietin-like protein 2 induces proinflammatory responses in peritoneal cells

Masato Umikawa, Asako Umikawa, Tsuyoshi Asato, Kimiko Takei, Goro Matsuzaki, Ken Ichi Kariya, Chengcheng Zhang

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Monocytes and macrophages are important effectors and regulators of inflammation, and both their differentiation and activation are regulated strictly in response to environmental cues. Angiopoietin-like protein 2 (Angptl2) is a multifaceted protein, displaying many physiological and pathological functions in inflammation, angiogenesis, hematopoiesis, and tumor development. Although recent studies implicate Angptl2 in chronic inflammation, the mechanisms of inflammation caused by Angptl2 remain unclear. The purpose of the present study was to elucidate the role of Angptl2 in inflammation by understanding the effects of Angptl2 on monocytes/macrophages. We showed that Angptl2 directly activates resident murine peritoneal monocytes and macrophages and induces a drastic upregulation of the transcription of several inflammatory genes including nitric oxide synthase 2 and prostaglandin-endoperoxide synthase 2, and several proinflammatory cytokine genes such as interleukin (IL)-1β, IL-6, TNFα, and CSF2, along with activation of ERK, JNK, p38, and nuclear factor kappa B signaling pathways. Concordantly, proinflammatory cytokines IL-1β, IL-6, TNFα, and GM-CSF, were rapidly elevated from murine peritoneal monocytes and macrophages. These results demonstrate a novel role for Angptl2 in inflammation via the direct activation of peritoneal monocytes and macrophages.

Original languageEnglish (US)
Pages (from-to)235-241
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume467
Issue number2
DOIs
StatePublished - Nov 13 2015

Keywords

  • Cytokine production
  • Inflammation
  • Macrophage
  • Monocyte
  • Peritoneal cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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