Angiotensin II regulates adrenal vascular tone through zona glomerulosa cell-derived EETs and DHETs

Phillip G. Kopf, Kathryn M. Gauthier, David X. Zhang, J R Falck, William B. Campbell

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Elevated concentrations of aldosterone are associated with several cardiovascular diseases. Angiotensin II (Ang II) increases aldosterone secretion and adrenal blood flow. This concurrent increase in steroidogenesis and adrenal blood flow is not understood. We investigated the role of zona glomerulosa (ZG) cells in the regulation of vascular tone of bovine adrenal cortical arteries by Ang II. ZG cells enhanced endothelium-dependent relaxations to Ang II. The ZG cell-dependent relaxations to Ang II were unchanged by removing the endothelium-dependent response to Ang II. These ZG cell-mediated relaxations were ablated by cytochrome P450 inhibition, epoxyeicosatrienoic acid (EET) antagonism, and potassium channel blockade. Analysis of ZG cell EET production by liquid chromatography/mass spectrometry demonstrated an increase in EETs and dihydroxyeicosatrienoic acids with Ang II stimulation. These EETs and dihydroxyeicosatrienoic acids produced similar concentration-dependent relaxations of adrenal arteries, which were attenuated by EET antagonism. Whole-cell potassium currents of adrenal artery smooth muscle cells were increased by Ang II stimulation in the presence of ZG cells but decreased in the absence of ZG cells. This increase in potassium current was abolished by iberiotoxin. Similarly, 14,15-EET induced concentration-dependent increases in potassium current, which was abolished by iberiotoxin. ZG cell aldosterone release was not directly altered by EETs. These data suggest that Ang II stimulates ZG cells to release EETs and dihydroxyeicosatrienoic acids, resulting in potassium channel activation and relaxation of adrenal arteries. This provides a mechanism by which Ang II concurrently increases adrenal blood flow and steroidogenesis.

Original languageEnglish (US)
Pages (from-to)323-329
Number of pages7
JournalHypertension
Volume57
Issue number2
DOIs
StatePublished - Feb 2011

Fingerprint

Zona Glomerulosa
Angiotensin II
Blood Vessels
Acids
Arteries
Aldosterone
Potassium
Potassium Channels
Endothelium
Liquid Chromatography
Cytochrome P-450 Enzyme System
Smooth Muscle Myocytes
Mass Spectrometry
Cardiovascular Diseases

Keywords

  • adrenal cortex
  • angiotensin II
  • epoxyeicosatrienoic acid
  • hyperpolarizing factor
  • potassium channel

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Angiotensin II regulates adrenal vascular tone through zona glomerulosa cell-derived EETs and DHETs. / Kopf, Phillip G.; Gauthier, Kathryn M.; Zhang, David X.; Falck, J R; Campbell, William B.

In: Hypertension, Vol. 57, No. 2, 02.2011, p. 323-329.

Research output: Contribution to journalArticle

Kopf, Phillip G. ; Gauthier, Kathryn M. ; Zhang, David X. ; Falck, J R ; Campbell, William B. / Angiotensin II regulates adrenal vascular tone through zona glomerulosa cell-derived EETs and DHETs. In: Hypertension. 2011 ; Vol. 57, No. 2. pp. 323-329.
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