Anion dependence of rabbit medullary collecting duct acidification

D. K. Stone, D. W. Seldin, J. P. Kokko, H. R. Jacobson

Research output: Contribution to journalArticle

78 Scopus citations

Abstract

Rabbit medullary collecting duct (MCD) acidification has been demonstrated to occur by means of a sodium-indpendent, aldosterone-stimulated mechanism. We have examined the anionic dependence of this process by means of the isolated perfused tubule technique. Total replacement of perfusate chloride with gluconate enhanced tubular bicarbonate reabsorption (JHCO3), from a basal rate of 10.7 ± 1.0 pmol.mm-1.min-1 to a rate of 15.01 ± 1.0 pmol.mm-1.min-1. Removal of bath chloride, with and without removal of perfusate chloride completely abolished acidification. Bath, but not luminal 4-acetamido-4' isothiocyano-2,2'-disulfonic stilbene provoked a marked decrease in JHCO3 from 10.1 ± 1.2 pmol.mm-1.min-1 to 2.3 ± 0.3 pmol.mm-1.min-1. Measurement of chloride reabsorptive rate (JCl) revealed colinearity between JHCO3 (9.18 ± 0.9 pmol.mm-1.min-1) and JCl (9.75 ± 1.18 pmol.mm-1.min-1). We propose a model of mammalian distal nephron acidification in which (a) cellular base exit is effected by means of a basolateral membrane Cl-base exchanger and (b) net electroneutrality of electrogenic proton secretion is maintained by the parallel movement of an anionic species, functionally chloride.

Original languageEnglish (US)
Pages (from-to)1505-1508
Number of pages4
JournalJournal of Clinical Investigation
Volume71
Issue number5
DOIs
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Anion dependence of rabbit medullary collecting duct acidification'. Together they form a unique fingerprint.

  • Cite this

    Stone, D. K., Seldin, D. W., Kokko, J. P., & Jacobson, H. R. (1983). Anion dependence of rabbit medullary collecting duct acidification. Journal of Clinical Investigation, 71(5), 1505-1508. https://doi.org/10.1172/JCI110905