Anisotropic processing of laser speckle images improves spatiotemporal resolution

Abhishek Rege, Janaka Senarathna, Nan Li, Nitish V. Thakor

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


Laser speckle contrast imaging (LSCI) is a full field optical imaging technique, capable of imaging blood flow without the introduction of any exogenous dyes. Spatial and temporal resolution in LSCI images depend on how pixels are chosen from the raw image stack for contrast processing. However, all processing schemes are based on isotropic treatment of the spatial neighborhood about each pixel, restricting further improvement in spatiotemporal resolution and image quality. We present a novel spatiotemporal processing scheme for LSCI where the spatial neighborhood is anisotropic, that is, restricted along a specific direction that matches direction of blood flow. The technique allows for a significant increase in temporal resolution, from conventionally used 40or 80 frames to just three frames; while simultaneously achieving 23 and 47 higher signal-to-noise ratios over concurrent spatiotemporal schemes, when imaging rapid and slow functional changes in blood flow, respectively. We present the concept, justification, and performance evaluation of the novel scheme and demonstrate its suitability for imaging rapid changes in blood flow. Anisotropic LSCI was able to monitor the heart rate associated fluctuations in intravascular blood flow and showed them to be as high as 28 of the mean.

Original languageEnglish (US)
Article number612790
Pages (from-to)1272-1280
Number of pages9
JournalIEEE Transactions on Biomedical Engineering
Issue number5
StatePublished - May 2012
Externally publishedYes


  • Blood flow
  • brain vasculature
  • laser speckle contrast imaging
  • microvessel imaging

ASJC Scopus subject areas

  • Biomedical Engineering


Dive into the research topics of 'Anisotropic processing of laser speckle images improves spatiotemporal resolution'. Together they form a unique fingerprint.

Cite this