ANKRD16 prevents neuron loss caused by an editing-defective tRNA synthetase

My Nuong Vo, Markus Terrey, Jeong Woong Lee, Bappaditya Roy, James J. Moresco, Litao Sun, Hongjun Fu, Qi Liu, Thomas G. Weber, John R. Yates, Kurt Fredrick, Paul Schimmel, Susan L. Ackerman

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Editing domains of aminoacyl tRNA synthetases correct tRNA charging errors to maintain translational fidelity. A mutation in the editing domain of alanyl tRNA synthetase (AlaRS) in Aarssti mutant mice results in an increase in the production of serine-mischarged tRNAAla and the degeneration of cerebellar Purkinje cells. Here, using positional cloning, we identified Ankrd16, a gene that acts epistatically with the Aarssti mutation to attenuate neurodegeneration. ANKRD16, a vertebrate-specific protein that contains ankyrin repeats, binds directly to the catalytic domain of AlaRS. Serine that is misactivated by AlaRS is captured by the lysine side chains of ANKRD16, which prevents the charging of serine adenylates to tRNAAla and precludes serine misincorporation in nascent peptides. The deletion of Ankrd16 in the brains of Aarssti/sti mice causes widespread protein aggregation and neuron loss. These results identify an amino-acid-accepting co-regulator of tRNA synthetase editing as a new layer of the machinery that is essential to the prevention of severe pathologies that arise from defects in editing.

Original languageEnglish (US)
Pages (from-to)510-515
Number of pages6
JournalNature
Volume557
Issue number7706
DOIs
StatePublished - May 24 2018

ASJC Scopus subject areas

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    Vo, M. N., Terrey, M., Lee, J. W., Roy, B., Moresco, J. J., Sun, L., Fu, H., Liu, Q., Weber, T. G., Yates, J. R., Fredrick, K., Schimmel, P., & Ackerman, S. L. (2018). ANKRD16 prevents neuron loss caused by an editing-defective tRNA synthetase. Nature, 557(7706), 510-515. https://doi.org/10.1038/s41586-018-0137-8