Annexin A3 regulates early blood vessel formation

Stryder M. Meadows, Ondine Cleaver

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Annexins are a large family of calcium binding proteins that associate with cell membrane phospholipids and are involved in various cellular processes including endocytosis, exocytosis and membrane-cytoskeletal organization. Despite studies on numerous Annexin proteins, the function of Annexin A3 (Anxa3) is largely unknown. Our studies identify Anxa3 as a unique marker of the endothelial and myeloid cell lineages of Xenopus laevis during development. Anxa3 transcripts are also detected in endothelial cells (ECs) of zebrafish and mouse embryos, suggesting an important evolutionary function during formation of blood vessels. Indeed, Anxa3 loss-of-function experiments in frog embryos reveal its critical role during the morphogenesis of early blood vessels, as angioblasts in MO injected embryos fail to form vascular cords. Furthermore, in vitro experiments in mammalian cells identify a role for Anxa3 in EC migration. Our results are the first to reveal an in vivo function for Anxa3 during vascular development and represent a previously unexplored aspect of annexin biology.

Original languageEnglish (US)
Article numbere0132580
JournalPLoS One
Volume10
Issue number7
DOIs
StatePublished - Jul 16 2015

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Annexin A3
Annexins
Blood vessels
blood vessels
Blood Vessels
embryo (animal)
Embryonic Structures
Endothelial Cells
endothelial cells
Endothelial cells
calcium-binding proteins
exocytosis
Xenopus laevis
endocytosis
Danio rerio
cell movement
Calcium-Binding Proteins
morphogenesis
Exocytosis
cell membranes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Annexin A3 regulates early blood vessel formation. / Meadows, Stryder M.; Cleaver, Ondine.

In: PLoS One, Vol. 10, No. 7, e0132580, 16.07.2015.

Research output: Contribution to journalArticle

Meadows, Stryder M. ; Cleaver, Ondine. / Annexin A3 regulates early blood vessel formation. In: PLoS One. 2015 ; Vol. 10, No. 7.
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