Annexin1 regulates the erythroid differentiation through ERK signaling pathway

Xiao Fang Huo, Jun Wu Zhang

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

K562 cells can be used as a model of erythroid differentiation on being induced by hemin. We found that the level of annexin1 gene expression was notably increased during this indicated process. To test the hypothesis that annexin1 can regulate erythropoiesis, K562 cell clones in which annexin1 was stably increased and was knocked down by RNAi were established, respectively. With analysis by hemoglobin quantification, benzidine staining, and marker gene expression profile determination, we confirmed that hemin-induced erythroid differentiation of K562 cells was modestly stimulated by overexpression of annexin1 while it was significantly blocked by knock down of annexin1. Further studies revealed that the mechanisms of annexin1 regulation of the erythroid differentiation was partially related to the increased ERK phosphorylation and expression of p21cip/waf, since specific inhibitor of MEK blocked the function of annexin1 in erythroid differentiation. We concluded that annexin1 exerted its erythropoiesis regulating effect by ERK pathway.

Original languageEnglish (US)
Pages (from-to)1346-1352
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume331
Issue number4
DOIs
StatePublished - Jun 17 2005

Keywords

  • Annexin1
  • ERK signaling pathway
  • Erythroid differentiation
  • K562 cells

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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