Abstract
Metabolic chemical reports have fundamentally changed the way researchers study glycosylation. However, when administered as per-O-acetylated sugars, reporter molecules can participate in nonspecific chemical labeling of cysteine residues termed S-glycosylation. Without detailed proteomic analyses, these labeling events can be indistinguishable from bona fide enzymatic labeling convoluting experimental results. Here, we report a solution in the synthesis and characterization of two reporter molecules functionalized at the anomeric position with hexanoic acid: 1-Hex-GlcNAlk and 1-Hex-6AzGlcNAc. Both reporters exhibit robust labeling over background with negligible amounts of nonspecific chemical labeling in cell lysates. This strategy serves as a template for the design of future reporter molecules allowing for more reliable interpretation of results.
Original language | English (US) |
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Pages (from-to) | 1924-1929 |
Number of pages | 6 |
Journal | ACS chemical biology |
Volume | 16 |
Issue number | 10 |
DOIs | |
State | Published - Oct 15 2021 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine