Anti-CD11b monoclonal antibody improves myocardial function after six hours of hypothermic storage

Joseph M. Forbess, Takeshi Hiramatsu, Fumikazu Nomura, Takuya Miura, G. King Farrington, Karen Sokolowski, Mark Bree, John E. Mayer

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Background.: The shortage of pediatric heart donors often necessitates considerable travel time and, as a result, prolonged donor heart ischemia. This excessive hypothermic storage may contribute markedly to myocardial dysfunction in the recipient. Methods.: We investigated the role of leukocyte-endothelial interactions in this dysfunction in an isolated, immature (mean age, 11.8 ± 1.6 days) swine heart model using a monoclonal antibody against a leukocyte adhesion molecule. We studied a total of 20 hearts subjected to 6 hours of cardioplegic arrest at 4°C. Group M1/70 (n = 6) received at reperfusion 15 μg/mL of a monoclonal antibody F(ab′)2 fragment to CD11b, the α-subunit of the leukocyte adhesion molecule Mac-1. Group MB10.6 (n = 8) received 15 μg/mL of the swine unreactive F(ab′)2 MB10.6, and the third group received saline vehicle. Results.: Administration of M1/70 resulted in improved postischemic recovery of ventricular function compared with the two control groups (p < 0.05). Conclusions.: These data implicate leukocyte-endothelial interactions mediated by the leukocyte adhesion molecule CD11b in myocardial dysfunction after long-term hypothermic ischemia. Specific antiadhesion strategies such as this may safely extend storage time for pediatric donor hearts.

Original languageEnglish (US)
Pages (from-to)1238-1244
Number of pages7
JournalThe Annals of Thoracic Surgery
Volume60
Issue number5
DOIs
StatePublished - Nov 1995

ASJC Scopus subject areas

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine

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    Forbess, J. M., Hiramatsu, T., Nomura, F., Miura, T., Farrington, G. K., Sokolowski, K., Bree, M., & Mayer, J. E. (1995). Anti-CD11b monoclonal antibody improves myocardial function after six hours of hypothermic storage. The Annals of Thoracic Surgery, 60(5), 1238-1244. https://doi.org/10.1016/0003-4975(95)00618-U