Anti-epiligrin cicatricial pemphigoid and epidermolysis bullosa acquisita: Differentiation by use of indirect immunofluorescence microscopy

Robert M. Vodegel, Marcelus C J M De Jong, Hendri H. Pas, Kim B. Yancey, Marcel F. Jonkman

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Binding of autoantibodies to laminin 5 and type VII collagen causes anti-epiligrin cicatricial pemphigoid and epidermolysis bullosa acquisita, respectively. Differentiation between these two dermal-binding autoimmune bullous dermatoses is not yet possible by indirect immunofluorescence microscopy. In this study we tested whether two recently described immunofluorescence techniques, "knockout" skin substrate and fluorescent overlay antigen mapping, can differentiate between anti-epiligrin cicatricial pemphigoid and epidermolysis bullosa acquisita. A total of 10 sera were tested: 4 with antilaminin 5, and 6 with antitype VII collagen autoantibodies, as characterized by either immunoblot or immunoprecipitation analysis. Differentiation between anti-epiligrin cicatricial pemphigoid and epidermolysis bullosa acquisita was possible in all 10 sera by indirect immunofluorescence using either knockout skin substrate or fluorescent overlay antigen mapping technique.

Original languageEnglish (US)
Pages (from-to)542-547
Number of pages6
JournalJournal of the American Academy of Dermatology
Volume48
Issue number4
DOIs
StatePublished - Apr 1 2003

ASJC Scopus subject areas

  • Dermatology

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