Anti-inflammatory and antiproliferating properties of allylamines

U. E. Berry, T. S. Choi, S. C. Geen, N. B. Silverberg, W. L. Lee, A. R. Shalita

Research output: Contribution to journalArticle

Abstract

Terbinafme (TB, SF86-327), a synthetic allylamine, is a broad-spectrum, orally active antifungal. Cytospin data showed that TB-treated (10-50 ug/ml for 30 min at 37 °C) human polymorphonuclear leukocytes (PMNs) displayed nuclear rounding with loss of lobulations and a reduction in the cytoplasmic to nuclear ratio. Similar cytoplasmic to nuclear ratio reductions were also demonstrated in TB-treated peripheral blood lymphocytes (PBLs). TB-treated PMNs (at > 25 ng/ml) exhibited normal respiratory burst activity measured by Superoxide anion production and chemiluminescence (CL), but showed reduced chemotaxis (CT). TB significantly (p <0.01-0.05) inhibited PMN and PBL adhesion to human microvascular endothelium (HMEC-1). The TB-treated leukocytes were stimulated by chemotactic factors or mitogens, and cell adhesion was measured by uptake of rose bengal stain using an ELISA reader. TB analogues (85-190 or naftifine) produced similar dose-dependent inhibitory effects on CT and cell adhesion to HMEC-1, the latter also inhibited CL. Cell cycle flow cytometric analysis showed doseand time-dependent alterations in Gl and/or G2+M phase(s) consistent with cell cycle and mitotic blockade in mitogen-activated PBLs and a promyelocytic cell line (HL60). Our data collectively demonstrates morphological and functional differences among allylaminetreated cells, which may explain many of the antiinflammatory and antiproliferation effects.

Original languageEnglish (US)
Pages (from-to)143
Number of pages1
JournalSkin Pharmacology
Volume9
Issue number2
StatePublished - 1996

Fingerprint

Allylamine
Anti-Inflammatory Agents
Chemotaxis
Lymphocytes
Luminescence
Mitogens
Cell Adhesion
Cell Cycle
Rose Bengal
Respiratory Burst
G2 Phase
Chemotactic Factors
Superoxides
Cell Division
Endothelium
Neutrophils
Leukocytes
Coloring Agents
Enzyme-Linked Immunosorbent Assay
Cell Line

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Dermatology
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Berry, U. E., Choi, T. S., Geen, S. C., Silverberg, N. B., Lee, W. L., & Shalita, A. R. (1996). Anti-inflammatory and antiproliferating properties of allylamines. Skin Pharmacology, 9(2), 143.

Anti-inflammatory and antiproliferating properties of allylamines. / Berry, U. E.; Choi, T. S.; Geen, S. C.; Silverberg, N. B.; Lee, W. L.; Shalita, A. R.

In: Skin Pharmacology, Vol. 9, No. 2, 1996, p. 143.

Research output: Contribution to journalArticle

Berry, UE, Choi, TS, Geen, SC, Silverberg, NB, Lee, WL & Shalita, AR 1996, 'Anti-inflammatory and antiproliferating properties of allylamines', Skin Pharmacology, vol. 9, no. 2, pp. 143.
Berry UE, Choi TS, Geen SC, Silverberg NB, Lee WL, Shalita AR. Anti-inflammatory and antiproliferating properties of allylamines. Skin Pharmacology. 1996;9(2):143.
Berry, U. E. ; Choi, T. S. ; Geen, S. C. ; Silverberg, N. B. ; Lee, W. L. ; Shalita, A. R. / Anti-inflammatory and antiproliferating properties of allylamines. In: Skin Pharmacology. 1996 ; Vol. 9, No. 2. pp. 143.
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