Antibodies which block anti-myelin basic protein antibodies associated with development of experimental autoimmune encephalomyelitis in Wistar rats

Aldo Alejandro Vilcaes, Alicia L. Degano, Pablo H H López, Gustavo A. Nores, German A. Roth

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: In sera from normal rats and from rats injected with whole myelin in complete Freund adjuvant to induce EAE we study the presence of antibodies capable to inhibit the reactivity of autoantibodies directed to myelin basic protein (MBP). Methods: Sera from rats that developed or not clinical signs of EAE were obtained previously to immunization, at acute stage of the disease and when the animals were completely recuperated, and chromatographied on a protein G-Sepharose column to obtain the retained (IgG) fractions. Then these fractions were depleted of anti-MBP reactivity by affinity chromatography and the ability of these depleted sera to block the reactivity of anti-MBP IgG antibodies was analyzed by an immunoblot technique. Results: IgG fractions from preimmune sera inhibited the anti-MBP IgG reactivity associated to EAE. The analysis of sick EAE animals showed that the inhibitory activity faded away with the onset of the clinical signs but returned at its maximum value during the spontaneous remission. Animals that never developed clinical EAE did not show changes in the level of inhibitory activity that was similar to that observed in the preimmune sera. Conclusions: The presence of IgG antibodies blocking the anti-MBP IgG reactivity correlates with the development of the clinical signs of EAE.

Original languageEnglish (US)
Pages (from-to)31-36
Number of pages6
JournalJournal of Neuroimmunology
Volume164
Issue number1-2
DOIs
StatePublished - Jul 1 2005
Externally publishedYes

Fingerprint

Myelin Basic Protein
Autoimmune Experimental Encephalomyelitis
Wistar Rats
Immunoglobulin G
Antibodies
Serum
Spontaneous Remission
Blocking Antibodies
Freund's Adjuvant
Acute Disease
Myelin Sheath
Affinity Chromatography
Autoantibodies
Sepharose
Anti-Idiotypic Antibodies
Immunization
Proteins

Keywords

  • Autoantibodies
  • Autoimmunity
  • Encephalomyelitis
  • Myelin basic protein

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Antibodies which block anti-myelin basic protein antibodies associated with development of experimental autoimmune encephalomyelitis in Wistar rats. / Vilcaes, Aldo Alejandro; Degano, Alicia L.; López, Pablo H H; Nores, Gustavo A.; Roth, German A.

In: Journal of Neuroimmunology, Vol. 164, No. 1-2, 01.07.2005, p. 31-36.

Research output: Contribution to journalArticle

@article{4e7f266a354940f8b32116d9c06980b9,
title = "Antibodies which block anti-myelin basic protein antibodies associated with development of experimental autoimmune encephalomyelitis in Wistar rats",
abstract = "Objective: In sera from normal rats and from rats injected with whole myelin in complete Freund adjuvant to induce EAE we study the presence of antibodies capable to inhibit the reactivity of autoantibodies directed to myelin basic protein (MBP). Methods: Sera from rats that developed or not clinical signs of EAE were obtained previously to immunization, at acute stage of the disease and when the animals were completely recuperated, and chromatographied on a protein G-Sepharose column to obtain the retained (IgG) fractions. Then these fractions were depleted of anti-MBP reactivity by affinity chromatography and the ability of these depleted sera to block the reactivity of anti-MBP IgG antibodies was analyzed by an immunoblot technique. Results: IgG fractions from preimmune sera inhibited the anti-MBP IgG reactivity associated to EAE. The analysis of sick EAE animals showed that the inhibitory activity faded away with the onset of the clinical signs but returned at its maximum value during the spontaneous remission. Animals that never developed clinical EAE did not show changes in the level of inhibitory activity that was similar to that observed in the preimmune sera. Conclusions: The presence of IgG antibodies blocking the anti-MBP IgG reactivity correlates with the development of the clinical signs of EAE.",
keywords = "Autoantibodies, Autoimmunity, Encephalomyelitis, Myelin basic protein",
author = "Vilcaes, {Aldo Alejandro} and Degano, {Alicia L.} and L{\'o}pez, {Pablo H H} and Nores, {Gustavo A.} and Roth, {German A.}",
year = "2005",
month = "7",
day = "1",
doi = "10.1016/j.jneuroim.2005.03.008",
language = "English (US)",
volume = "164",
pages = "31--36",
journal = "Journal of Neuroimmunology",
issn = "0165-5728",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Antibodies which block anti-myelin basic protein antibodies associated with development of experimental autoimmune encephalomyelitis in Wistar rats

AU - Vilcaes, Aldo Alejandro

AU - Degano, Alicia L.

AU - López, Pablo H H

AU - Nores, Gustavo A.

AU - Roth, German A.

PY - 2005/7/1

Y1 - 2005/7/1

N2 - Objective: In sera from normal rats and from rats injected with whole myelin in complete Freund adjuvant to induce EAE we study the presence of antibodies capable to inhibit the reactivity of autoantibodies directed to myelin basic protein (MBP). Methods: Sera from rats that developed or not clinical signs of EAE were obtained previously to immunization, at acute stage of the disease and when the animals were completely recuperated, and chromatographied on a protein G-Sepharose column to obtain the retained (IgG) fractions. Then these fractions were depleted of anti-MBP reactivity by affinity chromatography and the ability of these depleted sera to block the reactivity of anti-MBP IgG antibodies was analyzed by an immunoblot technique. Results: IgG fractions from preimmune sera inhibited the anti-MBP IgG reactivity associated to EAE. The analysis of sick EAE animals showed that the inhibitory activity faded away with the onset of the clinical signs but returned at its maximum value during the spontaneous remission. Animals that never developed clinical EAE did not show changes in the level of inhibitory activity that was similar to that observed in the preimmune sera. Conclusions: The presence of IgG antibodies blocking the anti-MBP IgG reactivity correlates with the development of the clinical signs of EAE.

AB - Objective: In sera from normal rats and from rats injected with whole myelin in complete Freund adjuvant to induce EAE we study the presence of antibodies capable to inhibit the reactivity of autoantibodies directed to myelin basic protein (MBP). Methods: Sera from rats that developed or not clinical signs of EAE were obtained previously to immunization, at acute stage of the disease and when the animals were completely recuperated, and chromatographied on a protein G-Sepharose column to obtain the retained (IgG) fractions. Then these fractions were depleted of anti-MBP reactivity by affinity chromatography and the ability of these depleted sera to block the reactivity of anti-MBP IgG antibodies was analyzed by an immunoblot technique. Results: IgG fractions from preimmune sera inhibited the anti-MBP IgG reactivity associated to EAE. The analysis of sick EAE animals showed that the inhibitory activity faded away with the onset of the clinical signs but returned at its maximum value during the spontaneous remission. Animals that never developed clinical EAE did not show changes in the level of inhibitory activity that was similar to that observed in the preimmune sera. Conclusions: The presence of IgG antibodies blocking the anti-MBP IgG reactivity correlates with the development of the clinical signs of EAE.

KW - Autoantibodies

KW - Autoimmunity

KW - Encephalomyelitis

KW - Myelin basic protein

UR - http://www.scopus.com/inward/record.url?scp=20444453949&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20444453949&partnerID=8YFLogxK

U2 - 10.1016/j.jneuroim.2005.03.008

DO - 10.1016/j.jneuroim.2005.03.008

M3 - Article

C2 - 15950291

AN - SCOPUS:20444453949

VL - 164

SP - 31

EP - 36

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

SN - 0165-5728

IS - 1-2

ER -