Antibody-dependent neutrophil-mediated killing of Acanthamoeba castellanii

G. L. Stewart, K. Shupe, I. Kim, R. E. Silvany, H. Alizadeh, James P McCulley, Jerry Y Niederkorn

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Neutrophils from naive rats lysed low numbers of Acanthamoeba castellanii in the presence of normal rat serum and significantly higher numbers of the parasite in the presence of serum from immunized rats. With normal rat serum, neutrophils from rats immunized with crude parasite extract or from naive rats killed similar percentages of A. caslellanii. However, neutrophils from immunized rats killed a significantly greater percentage of parasites in the presence of serum from immunized rats than was seen with any other combination of serum and neutrophils. The addition of supernatant from cultures of concanavalin A-stimulated rat spleen cells to incubations of the parasite in the presence of neutrophils from naive or immunized rats and immune serum resulted in the highest levels of amoebolysis seen in this study. This study has shown that neutrophils from naive rats or from rats immunized with A. castellanii antigen display a very limited amoebolytic capability which is significantly augmented in the presence of serum from immunized rats and further boosted by the addition of supernatant from Con A-stimulated rat spleen cell cultures.

Original languageEnglish (US)
Pages (from-to)739-742
Number of pages4
JournalInternational Journal for Parasitology
Volume24
Issue number5
DOIs
StatePublished - Aug 1994

Keywords

  • Acanthamoeba castellanii
  • concanavalin A
  • neutrophils

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Antibody-dependent neutrophil-mediated killing of Acanthamoeba castellanii'. Together they form a unique fingerprint.

Cite this