Antibody-resistant spread of vesicular stomatitis virus infection in cell lines of epithelial origin

M. G. Roth, R. W. Compans

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In MDCK cells, vesicular stomatitis virus (VSV) buds exclusively from the basolateral plasma membranes beneath tight junctions, whereas influenza virus forms only at the free apical surface. Anti-VSV antiserum did not prevent the formation of plaques on MDCK cell monolayers infected with VSV, whereas plaque formation in BHK-21 cells was completely inhibited by such antiserum. Under similar conditions, homologous antiserum completely prevented plaque formation by influenza virus on MDCK cells. In several other epithelioid cell lines, VSV also formed plaques in the presence of specific antiserum. These results suggest that VSV receptors are present on basolateral membranes in the cells studied and that junctional complexes present between cells may exclude antibody from intercellular spaces and thus permit the lateral spread of virus infection in the presence of neutralizing antibody.

Original languageEnglish (US)
Pages (from-to)547-550
Number of pages4
JournalJournal of Virology
Volume35
Issue number2
StatePublished - 1980

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Vesiculovirus
Vesicular Stomatitis
Virus Diseases
cell lines
Madin Darby Canine Kidney Cells
antiserum
Immune Sera
Cell Line
antibodies
Antibodies
Viruses
infection
Orthomyxoviridae
cells
Cell Membrane
Virus Receptors
Epithelioid Cells
tight junctions
intercellular spaces
Tight Junctions

ASJC Scopus subject areas

  • Immunology

Cite this

Antibody-resistant spread of vesicular stomatitis virus infection in cell lines of epithelial origin. / Roth, M. G.; Compans, R. W.

In: Journal of Virology, Vol. 35, No. 2, 1980, p. 547-550.

Research output: Contribution to journalArticle

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