Antigen-induced changes in phospholipid metabolism in antigen-binding B lymphocytes

C. D. Myers, M. K. Kriz, T. J. Sullivan, E. S. Vitetta

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Previous studies have demonstrated a marked change in the metabolism of phospholipids (PL) after activation of resting B lymphocytes with anti-immunoglobulin (anti-Ig). In this study we examined PL metabolism in highly purified trinitrophenyl (TNP)-binding B cells after their activation with various forms of TNP-carrier protein. Such cells show similar changes in PL metabolism when stimulated with either antigen or anti-Ig, i.e., increased incorporation of 32PO4 into phosphatidic acid and phosphatidyl inositol (PI) but not phosphatidyl choline, phosphatidyl ethanolamine, or phosphatidyl serine. We have demonstrated that these responses to antigen are TNP-specific and dose-related between 1 and 50 μg/ml, producing up to a 2.5-fold stimulation of 32PO4 incorporation into PI. The PL response is also directly related to the density of TNP on the carrier and can be augmented by additional cross-linking of the carrier protein. These data suggest that cross-linking of surface Ig by antigen induces a change in PL metabolism as an early event in B cell activation.

Original languageEnglish (US)
Pages (from-to)1705-1711
Number of pages7
JournalJournal of Immunology
Volume138
Issue number6
StatePublished - 1987

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Antigen-induced changes in phospholipid metabolism in antigen-binding B lymphocytes'. Together they form a unique fingerprint.

Cite this