Antigen-induced, tolerogenic CD11c+,CD11b+ dendritic cells are abundant in Peyer's patches during the induction of oral tolerance to type II collagen and suppress experimental collagen-induced arthritis

So Youn Min, Kyung Su Park, Mi La Cho, Jung Won Kang, Young Gyu Cho, Sue Yun Hwang, Min Jung Park, Chong Hyeon Yoon, Jun Ki Min, Sang Heon Lee, Sung Hwan Park, Ho Youn Kim

Research output: Contribution to journalArticle

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Abstract

Objective. Although oral tolerance is a well-known phenomenon, the role of dendritic cells (DCs) is not well characterized. This study was conducted to better understand the differential role played by each Peyer's patch DC subset in the induction of oral tolerance to type II collagen (CII) in murine collagen-induced arthritis (CIA). Methods. CII was fed 6 times to DBA/1 mice beginning 2 weeks before immunization, and the effect on arthritis was assessed. We compared the proportion of CD11c+,CD11b+ DCs and CD11c+,CD8α+ DCs in the Peyer's patches of CII-Fed tolerized and phosphate buffered saline-fed nontolerized mice after the induction of CIA. The immunosuppressive properties of each DC subset were determined using fluorescence-activated cell sorter analysis for intracellular interleukin-10 (IL-10) and IL-12 and mixed lymphocyte culture. The ability of each DC subset to induce CD4+,CD25+ T regulatory cells was also examined. Mice were injected with CII-pulsed CB11c+,CD11b+ DCs isolated from Peyer's patches of tolerized mice, and the effect on CIA was examined. Results. The severity of arthritis was significantly lower in tolerized mice. The proportion of CD11c+,CD11b+ DCs was increased in the Peyer's patches of tolerized mice and those DCs exhibited immunosuppressive characteristics, such as increased IL-10 production, inhibition of T cell proliferative responses to CII, and CD4+,CD25+ regulatory T cell induction. Furthermore, the CD11c+,CD11b+ DCs suppressed the severity of arthritis upon adoptive transfer. Conclusion. Our observations demonstrate that CD11c+,CD11b+ DCs, which are abundant in Peyer's patches during the induction of oral tolerance to CII, are crucial for the suppression of CIA and could be exploited for immunotherapy of autoimmune diseases.

Original languageEnglish (US)
Pages (from-to)887-898
Number of pages12
JournalArthritis and Rheumatism
Volume54
Issue number3
DOIs
StatePublished - Mar 2006

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Peyer's Patches
Experimental Arthritis
Collagen Type II
Dendritic Cells
Antigens
Arthritis
Regulatory T-Lymphocytes
Immunosuppressive Agents
Interleukin-10
Inbred DBA Mouse
Adoptive Transfer
Interleukin-12
Immunotherapy
Autoimmune Diseases
Immunization
Fluorescence
Phosphates
Lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Antigen-induced, tolerogenic CD11c+,CD11b+ dendritic cells are abundant in Peyer's patches during the induction of oral tolerance to type II collagen and suppress experimental collagen-induced arthritis. / Min, So Youn; Park, Kyung Su; Cho, Mi La; Kang, Jung Won; Cho, Young Gyu; Hwang, Sue Yun; Park, Min Jung; Yoon, Chong Hyeon; Min, Jun Ki; Lee, Sang Heon; Park, Sung Hwan; Kim, Ho Youn.

In: Arthritis and Rheumatism, Vol. 54, No. 3, 03.2006, p. 887-898.

Research output: Contribution to journalArticle

Min, So Youn ; Park, Kyung Su ; Cho, Mi La ; Kang, Jung Won ; Cho, Young Gyu ; Hwang, Sue Yun ; Park, Min Jung ; Yoon, Chong Hyeon ; Min, Jun Ki ; Lee, Sang Heon ; Park, Sung Hwan ; Kim, Ho Youn. / Antigen-induced, tolerogenic CD11c+,CD11b+ dendritic cells are abundant in Peyer's patches during the induction of oral tolerance to type II collagen and suppress experimental collagen-induced arthritis. In: Arthritis and Rheumatism. 2006 ; Vol. 54, No. 3. pp. 887-898.
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abstract = "Objective. Although oral tolerance is a well-known phenomenon, the role of dendritic cells (DCs) is not well characterized. This study was conducted to better understand the differential role played by each Peyer's patch DC subset in the induction of oral tolerance to type II collagen (CII) in murine collagen-induced arthritis (CIA). Methods. CII was fed 6 times to DBA/1 mice beginning 2 weeks before immunization, and the effect on arthritis was assessed. We compared the proportion of CD11c+,CD11b+ DCs and CD11c+,CD8α+ DCs in the Peyer's patches of CII-Fed tolerized and phosphate buffered saline-fed nontolerized mice after the induction of CIA. The immunosuppressive properties of each DC subset were determined using fluorescence-activated cell sorter analysis for intracellular interleukin-10 (IL-10) and IL-12 and mixed lymphocyte culture. The ability of each DC subset to induce CD4+,CD25+ T regulatory cells was also examined. Mice were injected with CII-pulsed CB11c+,CD11b+ DCs isolated from Peyer's patches of tolerized mice, and the effect on CIA was examined. Results. The severity of arthritis was significantly lower in tolerized mice. The proportion of CD11c+,CD11b+ DCs was increased in the Peyer's patches of tolerized mice and those DCs exhibited immunosuppressive characteristics, such as increased IL-10 production, inhibition of T cell proliferative responses to CII, and CD4+,CD25+ regulatory T cell induction. Furthermore, the CD11c+,CD11b+ DCs suppressed the severity of arthritis upon adoptive transfer. Conclusion. Our observations demonstrate that CD11c+,CD11b+ DCs, which are abundant in Peyer's patches during the induction of oral tolerance to CII, are crucial for the suppression of CIA and could be exploited for immunotherapy of autoimmune diseases.",
author = "Min, {So Youn} and Park, {Kyung Su} and Cho, {Mi La} and Kang, {Jung Won} and Cho, {Young Gyu} and Hwang, {Sue Yun} and Park, {Min Jung} and Yoon, {Chong Hyeon} and Min, {Jun Ki} and Lee, {Sang Heon} and Park, {Sung Hwan} and Kim, {Ho Youn}",
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T1 - Antigen-induced, tolerogenic CD11c+,CD11b+ dendritic cells are abundant in Peyer's patches during the induction of oral tolerance to type II collagen and suppress experimental collagen-induced arthritis

AU - Min, So Youn

AU - Park, Kyung Su

AU - Cho, Mi La

AU - Kang, Jung Won

AU - Cho, Young Gyu

AU - Hwang, Sue Yun

AU - Park, Min Jung

AU - Yoon, Chong Hyeon

AU - Min, Jun Ki

AU - Lee, Sang Heon

AU - Park, Sung Hwan

AU - Kim, Ho Youn

PY - 2006/3

Y1 - 2006/3

N2 - Objective. Although oral tolerance is a well-known phenomenon, the role of dendritic cells (DCs) is not well characterized. This study was conducted to better understand the differential role played by each Peyer's patch DC subset in the induction of oral tolerance to type II collagen (CII) in murine collagen-induced arthritis (CIA). Methods. CII was fed 6 times to DBA/1 mice beginning 2 weeks before immunization, and the effect on arthritis was assessed. We compared the proportion of CD11c+,CD11b+ DCs and CD11c+,CD8α+ DCs in the Peyer's patches of CII-Fed tolerized and phosphate buffered saline-fed nontolerized mice after the induction of CIA. The immunosuppressive properties of each DC subset were determined using fluorescence-activated cell sorter analysis for intracellular interleukin-10 (IL-10) and IL-12 and mixed lymphocyte culture. The ability of each DC subset to induce CD4+,CD25+ T regulatory cells was also examined. Mice were injected with CII-pulsed CB11c+,CD11b+ DCs isolated from Peyer's patches of tolerized mice, and the effect on CIA was examined. Results. The severity of arthritis was significantly lower in tolerized mice. The proportion of CD11c+,CD11b+ DCs was increased in the Peyer's patches of tolerized mice and those DCs exhibited immunosuppressive characteristics, such as increased IL-10 production, inhibition of T cell proliferative responses to CII, and CD4+,CD25+ regulatory T cell induction. Furthermore, the CD11c+,CD11b+ DCs suppressed the severity of arthritis upon adoptive transfer. Conclusion. Our observations demonstrate that CD11c+,CD11b+ DCs, which are abundant in Peyer's patches during the induction of oral tolerance to CII, are crucial for the suppression of CIA and could be exploited for immunotherapy of autoimmune diseases.

AB - Objective. Although oral tolerance is a well-known phenomenon, the role of dendritic cells (DCs) is not well characterized. This study was conducted to better understand the differential role played by each Peyer's patch DC subset in the induction of oral tolerance to type II collagen (CII) in murine collagen-induced arthritis (CIA). Methods. CII was fed 6 times to DBA/1 mice beginning 2 weeks before immunization, and the effect on arthritis was assessed. We compared the proportion of CD11c+,CD11b+ DCs and CD11c+,CD8α+ DCs in the Peyer's patches of CII-Fed tolerized and phosphate buffered saline-fed nontolerized mice after the induction of CIA. The immunosuppressive properties of each DC subset were determined using fluorescence-activated cell sorter analysis for intracellular interleukin-10 (IL-10) and IL-12 and mixed lymphocyte culture. The ability of each DC subset to induce CD4+,CD25+ T regulatory cells was also examined. Mice were injected with CII-pulsed CB11c+,CD11b+ DCs isolated from Peyer's patches of tolerized mice, and the effect on CIA was examined. Results. The severity of arthritis was significantly lower in tolerized mice. The proportion of CD11c+,CD11b+ DCs was increased in the Peyer's patches of tolerized mice and those DCs exhibited immunosuppressive characteristics, such as increased IL-10 production, inhibition of T cell proliferative responses to CII, and CD4+,CD25+ regulatory T cell induction. Furthermore, the CD11c+,CD11b+ DCs suppressed the severity of arthritis upon adoptive transfer. Conclusion. Our observations demonstrate that CD11c+,CD11b+ DCs, which are abundant in Peyer's patches during the induction of oral tolerance to CII, are crucial for the suppression of CIA and could be exploited for immunotherapy of autoimmune diseases.

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