Abstract
To investigate type II collagen (CII)-specific CD4+ T cell receptors involving in Collagen-induced arthritis (CIA) in DBA/1J mice as a model of rheumatoid arthritis in humans, TCRVβ usage in draining lymph nodes (dLNs) was assessed by flow cytometric analysis at 3, 5, and 8 weeks after bovine CII immunizations. In the early stage of CIA, the draining lymph node CD4+ T cells from CIA mice showed a higher proportion of CD4 + Vβ3+subsets compared with those from control mice. The CD4+ Vβ3+ T cells were specifically and primarily expanded by antigen-specific stimulation in in vitro culture of dLNs lymphocytes and splenocytes from CIA mice. In addition, CII-reactive response was observed when CD4+ Vβ3+ T cells were added to a non-responding T cell population. The adoptive transfer of CD4+ Vβ3+ T cells produced exaggerated arthritis compared with that in the control group. Our results indicate that CD4+Vβ3 + T cells, which were selectively expanded in dLN of CIA mice, play a pivotal role in CIA pathogenesis.
Original language | English (US) |
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Pages (from-to) | 204-212 |
Number of pages | 9 |
Journal | Journal of Clinical Immunology |
Volume | 26 |
Issue number | 3 |
DOIs | |
State | Published - May 2006 |
Keywords
- CD4 T cell
- Collagen-induced arthritis
- T cell receptor Vβ3
- Type II collagen
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology