Antigen-specific expansion of TCR Vβ3+CD4+ T cells in the early stage of collagen-induced arthritis and its arthritogenic role in DBA/1J mice

Jae Seon Lee, Mi La Cho, Joo Yeon Jhun, So Youn Min, Ji Hyeon Ju, Chong Hyeon Yoon, Jun Ki Min, Sung Hwan Park, Ho Youn Kim, Young Gyu Cho

Research output: Contribution to journalArticle

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Abstract

To investigate type II collagen (CII)-specific CD4+ T cell receptors involving in Collagen-induced arthritis (CIA) in DBA/1J mice as a model of rheumatoid arthritis in humans, TCRVβ usage in draining lymph nodes (dLNs) was assessed by flow cytometric analysis at 3, 5, and 8 weeks after bovine CII immunizations. In the early stage of CIA, the draining lymph node CD4+ T cells from CIA mice showed a higher proportion of CD4 + Vβ3+subsets compared with those from control mice. The CD4+ Vβ3+ T cells were specifically and primarily expanded by antigen-specific stimulation in in vitro culture of dLNs lymphocytes and splenocytes from CIA mice. In addition, CII-reactive response was observed when CD4+ Vβ3+ T cells were added to a non-responding T cell population. The adoptive transfer of CD4+ Vβ3+ T cells produced exaggerated arthritis compared with that in the control group. Our results indicate that CD4+Vβ3 + T cells, which were selectively expanded in dLN of CIA mice, play a pivotal role in CIA pathogenesis.

Original languageEnglish (US)
Pages (from-to)204-212
Number of pages9
JournalJournal of Clinical Immunology
Volume26
Issue number3
DOIs
StatePublished - May 2006

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Inbred DBA Mouse
Experimental Arthritis
T-Lymphocytes
Antigens
Lymph Nodes
Collagen Type II
Adoptive Transfer
T-Cell Antigen Receptor
Arthritis
Immunization
Rheumatoid Arthritis
Lymphocytes
Control Groups
Population

Keywords

  • CD4 T cell
  • Collagen-induced arthritis
  • T cell receptor Vβ3
  • Type II collagen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Antigen-specific expansion of TCR Vβ3+CD4+ T cells in the early stage of collagen-induced arthritis and its arthritogenic role in DBA/1J mice. / Lee, Jae Seon; Cho, Mi La; Jhun, Joo Yeon; Min, So Youn; Ju, Ji Hyeon; Yoon, Chong Hyeon; Min, Jun Ki; Park, Sung Hwan; Kim, Ho Youn; Cho, Young Gyu.

In: Journal of Clinical Immunology, Vol. 26, No. 3, 05.2006, p. 204-212.

Research output: Contribution to journalArticle

Lee, Jae Seon ; Cho, Mi La ; Jhun, Joo Yeon ; Min, So Youn ; Ju, Ji Hyeon ; Yoon, Chong Hyeon ; Min, Jun Ki ; Park, Sung Hwan ; Kim, Ho Youn ; Cho, Young Gyu. / Antigen-specific expansion of TCR Vβ3+CD4+ T cells in the early stage of collagen-induced arthritis and its arthritogenic role in DBA/1J mice. In: Journal of Clinical Immunology. 2006 ; Vol. 26, No. 3. pp. 204-212.
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