Antigen-specific HLA-restricted human T-cell lines - II. A GAT-specific T-cell line restricted by a determinant carried by an HLA-DQ molecule

Edward J. Ball, Peter Stastny

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Human T-cell lines responsive to the polypeptide antigens GAT and (T, G)-A--L were developed. They were specific for the priming antigens and required the participation of accessory cells matched for HLA-linked determinants, as shown in family studies. In panel studies, the ability of accessory cells to present antigen was shown to be associated with HLA-D-region antigens. However, the specificity of these determinants did not fully correspond to any HLA antigens as currently defined. One GAT-specific subline, derived by limiting dilution, utilized a restriction determinant associated with, but distinct from, the DQw3 (MB3) allospecificity. Blocking studies with mouse monoclonal antibodies indicated that this restriction determinant was carried by HLA-DQ molecules. The epitopes recognized in these molecules appear to be distinct from the alloantigenic determinants currently defined by serology.

Original languageEnglish (US)
Pages (from-to)547-564
Number of pages18
JournalImmunogenetics
Volume20
Issue number5
DOIs
StatePublished - Nov 1 1984

ASJC Scopus subject areas

  • Immunology
  • Genetics

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