Antigen-specific responses and ANA production in B6.Sle1b mice: A role for SAP

Paula Jennings, Alice Chan, Pamela Schwartzberg, Edward K. Wakeland, Dorothy Yuan

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

B6.Sle1b mice, which contain the Sle1b gene interval derived from lupus prone NZM2410 mice on a C57BL/6 background, present with gender-biased, highly penetrant anti-nuclear antibody (ANA) production. To obtain some insight into the possible induction mechanism of autoantibodies in these mice we compared antigen-specific T dependent (TD) and T independent (TI-II) responses between B6.Sle1b and B6 mice before the development of high ANA titers. Our results show that B6.Sle1b mice mount enhanced responses to a TI-II antigen. Additionally, the memory T cell response generated by a TD antigen also increased. This enhancement correlates with the greater ability of B cells from B6.Sle1b mice to present antigen to T cells. The SLAM Associated Protein (SAP) is critical for signaling of many of the molecules encoded by the SLAM/CD2 gene cluster, candidates for mediating the Sle1b phenotype; therefore, we also investigated the effect of sap deletion in these strains on the TD and TI-II responses as well as on ANA production. The results of these studies of responses to non-self-antigens provide further insight into the mechanism by which responses to self-antigens might be initiated in the context of specific genetic alterations.

Original languageEnglish (US)
Pages (from-to)345-353
Number of pages9
JournalJournal of Autoimmunity
Volume31
Issue number4
DOIs
StatePublished - Dec 1 2008

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Keywords

  • ANA
  • Antigen-specific responses
  • SAP
  • SLE

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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