Antigenic evidence for simultaneous expression of two different lipooligosaccharides by some strains of Haemophilus influenzae type b

C. C. Patrick, S. E. Pelzel, E. E. Miller, E. Haanes-Fritz, J. D. Radolf, P. A. Gulic, G. H. McCracken, E. J. Hansen

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Abstract

Isolates of Haemophilus influenzae type b (Hib) can be divided into three antigenic groups based on their reactivities with a set of two monoclonal antibodies (MAbs) directed against epitopes in the oligosaccharide region of Hib lipooligosaccharide (LOS) (P.A. Gulig, C.C. Patrick, L. Hermanstorfer, G.H. McCracken, Jr., and E.J. Hansen, Infect. Immun. 55:513-520, 1987). Approximately 24% of Hib strains react with both of these LOS-specific MAbs. Immunoprecipitation experiments involving several of these strains indicated that the epitopes recognized by these MAbs resided in two different LOS molecules, both of which were synthesized by these particular Hib strains. In addition, Western blot (immunoblot) analysis of proteinase K-treated cell extracts of these strains that had been subjected to sodium dodecyl sulfate-polyacrylamide gradient gel electrophoresis revealed two different LOS staining patterns when they were probed independently with the two MAbs. Colony blot radioimmunoassay of hundreds of colonies of one of these Hib strains showed that each colony bound both MAbs. Immune electron microscopy confirmed that individual cells of this same Hib strain expressed both types of LOS molecule at the same time. An antibody accessibility radioimmunoassay was used to show that different Hib strains of this type varied in the relative amounts of each of the two MAbs that they could bind to their cell surfaces. These findings indicate that some Hib strains can synthesize two antigenically distinct LOS molecules simultaneously.

Original languageEnglish (US)
Pages (from-to)1971-1978
Number of pages8
JournalInfection and Immunity
Volume57
Issue number7
StatePublished - 1989

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Haemophilus influenzae type b
Monoclonal Antibodies
Radioimmunoassay
Epitopes
Western Blotting
Endopeptidase K
lipid-linked oligosaccharides
Cell Extracts
Oligosaccharides
Immunoprecipitation
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Electron Microscopy
Staining and Labeling
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Patrick, C. C., Pelzel, S. E., Miller, E. E., Haanes-Fritz, E., Radolf, J. D., Gulic, P. A., ... Hansen, E. J. (1989). Antigenic evidence for simultaneous expression of two different lipooligosaccharides by some strains of Haemophilus influenzae type b. Infection and Immunity, 57(7), 1971-1978.

Antigenic evidence for simultaneous expression of two different lipooligosaccharides by some strains of Haemophilus influenzae type b. / Patrick, C. C.; Pelzel, S. E.; Miller, E. E.; Haanes-Fritz, E.; Radolf, J. D.; Gulic, P. A.; McCracken, G. H.; Hansen, E. J.

In: Infection and Immunity, Vol. 57, No. 7, 1989, p. 1971-1978.

Research output: Contribution to journalArticle

Patrick, C. C. ; Pelzel, S. E. ; Miller, E. E. ; Haanes-Fritz, E. ; Radolf, J. D. ; Gulic, P. A. ; McCracken, G. H. ; Hansen, E. J. / Antigenic evidence for simultaneous expression of two different lipooligosaccharides by some strains of Haemophilus influenzae type b. In: Infection and Immunity. 1989 ; Vol. 57, No. 7. pp. 1971-1978.
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abstract = "Isolates of Haemophilus influenzae type b (Hib) can be divided into three antigenic groups based on their reactivities with a set of two monoclonal antibodies (MAbs) directed against epitopes in the oligosaccharide region of Hib lipooligosaccharide (LOS) (P.A. Gulig, C.C. Patrick, L. Hermanstorfer, G.H. McCracken, Jr., and E.J. Hansen, Infect. Immun. 55:513-520, 1987). Approximately 24{\%} of Hib strains react with both of these LOS-specific MAbs. Immunoprecipitation experiments involving several of these strains indicated that the epitopes recognized by these MAbs resided in two different LOS molecules, both of which were synthesized by these particular Hib strains. In addition, Western blot (immunoblot) analysis of proteinase K-treated cell extracts of these strains that had been subjected to sodium dodecyl sulfate-polyacrylamide gradient gel electrophoresis revealed two different LOS staining patterns when they were probed independently with the two MAbs. Colony blot radioimmunoassay of hundreds of colonies of one of these Hib strains showed that each colony bound both MAbs. Immune electron microscopy confirmed that individual cells of this same Hib strain expressed both types of LOS molecule at the same time. An antibody accessibility radioimmunoassay was used to show that different Hib strains of this type varied in the relative amounts of each of the two MAbs that they could bind to their cell surfaces. These findings indicate that some Hib strains can synthesize two antigenically distinct LOS molecules simultaneously.",
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