Antigens in human monocytes. III. Use of monocytes in typing for HLA-D related (DR) antigens

P. Colbaugh, P. Stastny

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Monocyte preparations were obtained from the peripheral blood by adherence to plastic dishes. The number of monocytes obtained was significantly greater than the number of B cells recovered from similar samples. Monocytes could be frozen and thawed with relatively little loss in cell numbers or viability. Cytotoxicity tests were performed most reliably at 20°C. Under these conditions, autoantibodies were rare, and normal sera were consistently negative. Serologic reactions obtained with monocytes correlated well with the known specificities of the monocyte panel. Typing results in both normal and diseased populations were similar to results obtained with B lymphocytes. Moreover, monocyte cytotoxicity tests were more convenient and easier to read than similar tests performed with B cells.

Original languageEnglish (US)
Pages (from-to)871-874
Number of pages4
JournalTransplantation Proceedings
Volume10
Issue number4
StatePublished - Dec 1 1978

ASJC Scopus subject areas

  • Surgery
  • Transplantation

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