Antiphospholipid antibodies induce monocyte chemoattractant protein-1 in endothelial cells

Chul Soo Cho, Mi La Cho, Pojen P. Chen, So Youn Min, Sue Yun Hwang, Kyung Soo Park, Wan Uk Kim, Do June Min, Jun Ki Min, Sung Hwan Park, Ho Youn Kim

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40 Scopus citations

Abstract

The presence of antiphospholipid Ab is associated with increased risk of thrombosis. The monocyte-endothelial cell interaction has been suggested to play a key role at the site of vascular injury during thrombosis. Therefore, we tested the effect of anticardiolipin Abs (aCL) on the production of monocyte chemoattractant protein-1 (MCP-1) in HUVEC. We found that monoclonal aCL as well as IgG fractions from patients with antiphospholipid syndrome (APS-IgG) could induce the production of MCP-1 in HUVEC. The ability of IgG aCL to induce MCP-1 production could be abrogated by preabsorption with cardiolipin liposomes. Simultaneous addition of either monoclonal aCL or APS-IgG with IL-1β resulted in synergistic increase in MCP-1 production, whereas the addition of control IgG lacking aCL activity did not alter IL-1β-induced levels of MCP-1. MCP-1 mRNA expression was also up-regulated when HUVEC were incubated with either APS-IgG or monoclonal aCL, and down-regulated by the treatment of dexamethasone. In addition, we found that serum levels of MCP-1 in 76 systemic lupus erythematosus patients correlated well with the titers of IgG aCL. Collectively, these results indicate that aCL could promote endothelial cell-monocyte cross-talk by enhancing the endothelial production of MCP-1, thereby shifting the hemostatic balance toward the prothrombotic state of APS.

Original languageEnglish (US)
Pages (from-to)4209-4215
Number of pages7
JournalJournal of Immunology
Volume168
Issue number8
DOIs
StatePublished - Apr 15 2002

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Cho, C. S., Cho, M. L., Chen, P. P., Min, S. Y., Hwang, S. Y., Park, K. S., Kim, W. U., Min, D. J., Min, J. K., Park, S. H., & Kim, H. Y. (2002). Antiphospholipid antibodies induce monocyte chemoattractant protein-1 in endothelial cells. Journal of Immunology, 168(8), 4209-4215. https://doi.org/10.4049/jimmunol.168.8.4209