Antisense-Mediated Transcript Knockdown Triggers Premature Transcription Termination

Jong Sun Lee; Joshua T. Mendell

doi:10.1016/j.molcel.2019.12.011

Antisense-Mediated Transcript Knockdown Triggers Premature Transcription Termination

Jong Sun Lee, Joshua T. Mendell

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

Antisense oligonucleotide (ASO)-mediated transcript knockdown is commonly used to interrogate the function of long noncoding RNAs (lncRNAs). Here, Lee and Mendell demonstrate that ASOs induce cleavage of nascent transcripts and thereby trigger premature transcription termination. Thus, these reagents cannot be used to establish an RNA-mediated function for a lncRNA locus.

Original language	English (US)
Pages (from-to)	1044-1054.e3
Journal	Molecular cell
Volume	77
Issue number	5
DOIs	https://doi.org/10.1016/j.molcel.2019.12.011
State	Published - Mar 5 2020

Keywords

ASO
XRN2
antisense oligonucleotide
lncRNA
long noncoding RNA
torpedo mechanism
transcription termination

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/j.molcel.2019.12.011

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title = "Antisense-Mediated Transcript Knockdown Triggers Premature Transcription Termination",

abstract = "Antisense oligonucleotide (ASO)-mediated transcript knockdown is commonly used to interrogate the function of long noncoding RNAs (lncRNAs). Here, Lee and Mendell demonstrate that ASOs induce cleavage of nascent transcripts and thereby trigger premature transcription termination. Thus, these reagents cannot be used to establish an RNA-mediated function for a lncRNA locus.",

keywords = "ASO, XRN2, antisense oligonucleotide, lncRNA, long noncoding RNA, torpedo mechanism, transcription termination",

author = "Lee, {Jong Sun} and Mendell, {Joshua T.}",

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T1 - Antisense-Mediated Transcript Knockdown Triggers Premature Transcription Termination

AU - Lee, Jong Sun

AU - Mendell, Joshua T.

PY - 2020/3/5

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N2 - Antisense oligonucleotide (ASO)-mediated transcript knockdown is commonly used to interrogate the function of long noncoding RNAs (lncRNAs). Here, Lee and Mendell demonstrate that ASOs induce cleavage of nascent transcripts and thereby trigger premature transcription termination. Thus, these reagents cannot be used to establish an RNA-mediated function for a lncRNA locus.

AB - Antisense oligonucleotide (ASO)-mediated transcript knockdown is commonly used to interrogate the function of long noncoding RNAs (lncRNAs). Here, Lee and Mendell demonstrate that ASOs induce cleavage of nascent transcripts and thereby trigger premature transcription termination. Thus, these reagents cannot be used to establish an RNA-mediated function for a lncRNA locus.

KW - ASO

KW - XRN2

KW - antisense oligonucleotide

KW - lncRNA

KW - long noncoding RNA

KW - torpedo mechanism

KW - transcription termination

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AN - SCOPUS:85078807017

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JO - Molecular cell

JF - Molecular cell

IS - 5

ER -

Antisense-Mediated Transcript Knockdown Triggers Premature Transcription Termination

Abstract

Keywords

ASJC Scopus subject areas

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