Antisense-Mediated Transcript Knockdown Triggers Premature Transcription Termination

Jong Sun Lee, Joshua T. Mendell

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Antisense oligonucleotide (ASO)-mediated transcript knockdown is commonly used to interrogate the function of long noncoding RNAs (lncRNAs). Here, Lee and Mendell demonstrate that ASOs induce cleavage of nascent transcripts and thereby trigger premature transcription termination. Thus, these reagents cannot be used to establish an RNA-mediated function for a lncRNA locus.

Original languageEnglish (US)
Pages (from-to)1044-1054.e3
JournalMolecular cell
Volume77
Issue number5
DOIs
StatePublished - Mar 5 2020

Keywords

  • antisense oligonucleotide
  • ASO
  • lncRNA
  • long noncoding RNA
  • torpedo mechanism
  • transcription termination
  • XRN2

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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