Abstract
Antisera were raised against purified subunits of regulatory GTP-binding proteins (G proteins) and against synthetic peptides that correspond to defined regions of G proteins. Peptide antisera were generated that recognized all α or all β subunits from G(s), G(i), G(o), and transducin; others recognized only G(sα) or G(oα). Such cross-reaction or complete specificity for a given α subunit was not obtained when purified subunits were injected. Peptide antisera were used to identify G protein subunits in selected tissue membrane preparations by immunoblots.
Original language | English (US) |
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Pages (from-to) | 265-269 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 83 |
Issue number | 2 |
DOIs | |
State | Published - 1986 |
ASJC Scopus subject areas
- General