Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins

S. M. Mumby, R. A. Kahn, D. R. Manning, A. G. Gilman

Research output: Contribution to journalArticle

264 Scopus citations

Abstract

Antisera were raised against purified subunits of regulatory GTP-binding proteins (G proteins) and against synthetic peptides that correspond to defined regions of G proteins. Peptide antisera were generated that recognized all α or all β subunits from G(s), G(i), G(o), and transducin; others recognized only G(sα) or G(oα). Such cross-reaction or complete specificity for a given α subunit was not obtained when purified subunits were injected. Peptide antisera were used to identify G protein subunits in selected tissue membrane preparations by immunoblots.

Original languageEnglish (US)
Pages (from-to)265-269
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number2
DOIs
StatePublished - Jan 1 1986

ASJC Scopus subject areas

  • General

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