Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins

S. M. Mumby, R. A. Kahn, D. R. Manning, A. G. Gilman

Research output: Contribution to journalArticle

263 Citations (Scopus)

Abstract

Antisera were raised against purified subunits of regulatory GTP-binding proteins (G proteins) and against synthetic peptides that correspond to defined regions of G proteins. Peptide antisera were generated that recognized all α or all β subunits from G(s), G(i), G(o), and transducin; others recognized only G(sα) or G(oα). Such cross-reaction or complete specificity for a given α subunit was not obtained when purified subunits were injected. Peptide antisera were used to identify G protein subunits in selected tissue membrane preparations by immunoblots.

Original languageEnglish (US)
Pages (from-to)265-269
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number2
StatePublished - 1986

Fingerprint

GTP-Binding Proteins
Immune Sera
Carrier Proteins
Peptides
Transducin
Cross Reactions
Protein Subunits
Membranes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins. / Mumby, S. M.; Kahn, R. A.; Manning, D. R.; Gilman, A. G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 2, 1986, p. 265-269.

Research output: Contribution to journalArticle

@article{c32b01366b134da98c3b832c897481ef,
title = "Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins",
abstract = "Antisera were raised against purified subunits of regulatory GTP-binding proteins (G proteins) and against synthetic peptides that correspond to defined regions of G proteins. Peptide antisera were generated that recognized all α or all β subunits from G(s), G(i), G(o), and transducin; others recognized only G(sα) or G(oα). Such cross-reaction or complete specificity for a given α subunit was not obtained when purified subunits were injected. Peptide antisera were used to identify G protein subunits in selected tissue membrane preparations by immunoblots.",
author = "Mumby, {S. M.} and Kahn, {R. A.} and Manning, {D. R.} and Gilman, {A. G.}",
year = "1986",
language = "English (US)",
volume = "83",
pages = "265--269",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "2",

}

TY - JOUR

T1 - Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins

AU - Mumby, S. M.

AU - Kahn, R. A.

AU - Manning, D. R.

AU - Gilman, A. G.

PY - 1986

Y1 - 1986

N2 - Antisera were raised against purified subunits of regulatory GTP-binding proteins (G proteins) and against synthetic peptides that correspond to defined regions of G proteins. Peptide antisera were generated that recognized all α or all β subunits from G(s), G(i), G(o), and transducin; others recognized only G(sα) or G(oα). Such cross-reaction or complete specificity for a given α subunit was not obtained when purified subunits were injected. Peptide antisera were used to identify G protein subunits in selected tissue membrane preparations by immunoblots.

AB - Antisera were raised against purified subunits of regulatory GTP-binding proteins (G proteins) and against synthetic peptides that correspond to defined regions of G proteins. Peptide antisera were generated that recognized all α or all β subunits from G(s), G(i), G(o), and transducin; others recognized only G(sα) or G(oα). Such cross-reaction or complete specificity for a given α subunit was not obtained when purified subunits were injected. Peptide antisera were used to identify G protein subunits in selected tissue membrane preparations by immunoblots.

UR - http://www.scopus.com/inward/record.url?scp=2142858516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2142858516&partnerID=8YFLogxK

M3 - Article

VL - 83

SP - 265

EP - 269

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 2

ER -