TY - JOUR
T1 - Antisocial, an Intracellular Adaptor Protein, Is Required for Myoblast Fusion in Drosophila
AU - Chen, Elizabeth
AU - Olson, Eric N
N1 - Funding Information:
We thank Drs. Susan Abmayr, Micheal Bate, Mar Ruiz-Gómez, Dan Kiehart, and Hanh Nguyen for antibodies and cDNAs. We thank Akinao Nose and the Bloomington and Tübingen Drosophila Stock Centers for fly stocks, and Developmental Studies Hybridoma Bank for antibodies. We are grateful to Brian Pryce, Scott Herring, Caleb Roth, and Joseph Oberdorfer for excellent technical support. We thank James Richardson and the Pathology Core Facility at UT Southwestern for in situ hybridization of mouse embryos, Alisha Tizenor for graphics, and Jennifer Page for editorial assistance. We appreciate Dennis McKearin, Duojia Pan, and Keith Wharton for insightful discussions and critical reading of the manuscript. E.H.C. was supported by a postdoctoral fellowship from the Helen Hay Whitney Foundation. This work was supported by grants from the National Institutes of Health and the D.W. Reynolds Center for Clinical Cardiovascular Research to E.N.O.
PY - 2001/11
Y1 - 2001/11
N2 - Somatic muscle formation in Drosophila requires fusion of muscle founder cells with fusion-competent myoblasts. In a genetic screen for genes that control muscle development, we identified antisocial (ants), a gene that encodes an ankyrin repeat-, TPR repeat-, and RING finger-containing protein, required for myoblast fusion. In ants mutant embryos, founder cells and fusion-competent myoblasts are properly specified and patterned, but they are unable to form myotubes. ANTS, which is expressed specifically in founder cells, interacts with the cytoplasmic domain of Dumbfounded, a founder cell transmembrane receptor, and with Myoblast city, a cytoskeletal protein, both of which are also required for myoblast fusion. These findings suggest that ANTS functions as an intracellular adaptor protein that relays signals from Dumbfounded to the cytoskeleton during myoblast fusion.
AB - Somatic muscle formation in Drosophila requires fusion of muscle founder cells with fusion-competent myoblasts. In a genetic screen for genes that control muscle development, we identified antisocial (ants), a gene that encodes an ankyrin repeat-, TPR repeat-, and RING finger-containing protein, required for myoblast fusion. In ants mutant embryos, founder cells and fusion-competent myoblasts are properly specified and patterned, but they are unable to form myotubes. ANTS, which is expressed specifically in founder cells, interacts with the cytoplasmic domain of Dumbfounded, a founder cell transmembrane receptor, and with Myoblast city, a cytoskeletal protein, both of which are also required for myoblast fusion. These findings suggest that ANTS functions as an intracellular adaptor protein that relays signals from Dumbfounded to the cytoskeleton during myoblast fusion.
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U2 - 10.1016/S1534-5807(01)00084-3
DO - 10.1016/S1534-5807(01)00084-3
M3 - Article
C2 - 11709190
AN - SCOPUS:0035515458
SN - 1534-5807
VL - 1
SP - 705
EP - 715
JO - Developmental cell
JF - Developmental cell
IS - 5
ER -